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AdipoGen Life Sciences
IL-2 (human) (monomeric):Fc-KIH (human) (rec.)
330
CHF
CHF 330.00
In stock
AG-40B-0224-C01010 µgCHF 330.00
AG-40B-0224-30103 x 10 µgCHF 660.00
AG-40B-0224-C100100 µgCHF 1'320.00
Figure 1: IL-2 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0224) increases proliferation of CTLL-2 mouse cytotoxic T cells.
Method: Activity of IL-2 (human) (monomeric):Fc-KIH (human) (rec.) is measured using proliferation of CTLL-2 cells. CTLL-2 are grown in RPMI medium, glutamine, 10% FBS and different concentrations of IL-2 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0224) or control protein Fc-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0015). Cells proliferation is quantified using PMS/MTS (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega). ED50 of IL-2 (human) (monomeric):Fc-KIH (human) (rec.) is < 40pM.
Method: Activity of IL-2 (human) (monomeric):Fc-KIH (human) (rec.) is measured using proliferation of CTLL-2 cells. CTLL-2 are grown in RPMI medium, glutamine, 10% FBS and different concentrations of IL-2 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0224) or control protein Fc-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0015). Cells proliferation is quantified using PMS/MTS (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega). ED50 of IL-2 (human) (monomeric):Fc-KIH (human) (rec.) is < 40pM.
Figure 2: IL-2 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0224) and IL-2 (mouse) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0225) activate the IL-2 receptor using the IL-2 Bioassay (Promega JA2201). Method: IL-2 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0224), IL-2 (mouse) (monomeric):Fc-KIH (human) (rec.)
(Prod. No. AG-40B-0225) and Fc-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0015) are analyzed using the IL-2 Bioassay from Promega consisting of a genetically engineered cell line expressing the IL-2R. When IL-2 binds to its receptor (IL-2R), receptor-mediated pathway signaling induces luminescence that is measured with a luminometer.
Figure 3: Protein structure of IL-2 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0224).
| Product Details | |
|---|---|
| Synonyms | InVivoKine™; IL-2 (human) (monomeric):Fc Knobs-into-Holes (human) (rec.); Interleukin-2; T Cell Growth Factor; TCGF; Aldesleukin; Super-2 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
Human IL-2 (aa 21-153) is fused at the C-terminus to the Fc portion of human IgG1 (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity |
Human Mouse |
| Biological Activity |
Increases proliferation of CTLL‑2 mouse cytotoxic T cells with an ED50 of 2-5 pM (~0.17 to 0,42 ng/ml). |
| MW | ~48kDa and 28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg protein (LAL test). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml |
| Reconstitution | Reconstitute with 100μl endotoxin-free water. |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
Uniprot link P60568: IL-2 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.
IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Rα (CD25), IL-2Rβ and IL-2Rγ. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rβ and IL-2Rγ. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rβ and IL-2Rγ receptors. IL-2 is used in the treatment of metastatic cancer cells. Recently synergy has been observed between IL-2 based therapy and checkpoint blockade for cancer treatments. Cells which bear receptors for IL-2 stimulation are modulated by checkpoint inhibition either directly or through other lymphocytes, and lymphocytes which are effectors of checkpoint inhibition may respond to IL-2.
The protein IL-2 (human) (monomeric):Fc-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-2 (human):Fc Knobs sequence (synthesizing a protein of 45kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-2 (human) (monomeric):Fc-KIH (human) (rec.).
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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