AdipoGen Life Sciences

GLP-1R (human):Fc (LALA-PG)-KIH (human) (rec.)

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AG-40B-0272-C05050 µgCHF 440.00

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Figure 1: Schematic protein structure of GLP-1R (human):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0272).

Figure 2: GLP-1R (human):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0272) binds to its ligands Tirzepatide and Semaglutide.
Method: The ligands Tirzepatide (Prod. No. AG-CP3-0043) and Semaglutide (Prod. No. AG-CP3-0040) are coated on an ELISA plate at 500nM overnight at room temperature. GLP-1R (human):Fc (LALA-PG)-KIH (human) (rec.) or a control protein hIL-2-KIH (Prod. No. AG-40B-0263) (data not shown) is added starting at 160nM with a twofold serial dilution during one hour and then detected using a human Fc (HRP) antibody.

Specifications / Handling

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Product Details
Synonyms	InVivoKine™; GLP-1R (human) Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Glucagon-like Peptide 1 Receptor; GLP1R
Product Type	Protein
Properties
Source/Host	HEK 293 cells. Produced using animal component-free medium.
Sequence	Extracellular domain of human GLP-1R (aa 24-139) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).
Crossreactivity	Bovine Human Mouse Rat
Application	Binds to GLP-1, Semaglutide and Tirzepatide. Potential use for screening of new GLP-1R agonists.
Specificity	Human, Mouse, Rat, Bovine
MW	~65kDa and 28kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01 EU/μg protein (LAL test).
Concentration	After reconstitution: 1mg/ml
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	P43220
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018)
Other Product Data	Uniprot link P43220: GLP-1R (human)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Glucagon-like peptide 1 (GLP-1) is a 30-amino acid Incretin peptide hormone produced in the intestine by differential processing of proglucagon. Incretin hormones are gut peptides that are secreted after nutrient intake and stimulate insulin secretion together with hyperglycemia. GIP (glucose-dependent insulinotropic polypeptide) and GLP-1 (glucagon-like peptide-1) are the known incretin hormones from the upper (GIP, K cells) and lower (GLP-1, L cells) gut. GLP-1 receptors are abundant in the hypothalamus which contains projections to the hunger centers. GLP-1 decreases calorie intake, boosts feelings of satiety and encourages weight loss. GLP-1 and GIP hormones are used as drugs for weight loss in obese patients and also to lower blood sugar levels.

The protein GLP-1R (human):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the GLP-1R (human:Fc (LALA-PG) Knobs sequence (synthesizing a protein of 60kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein GLP-1R (human):Fc-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

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