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AdipoGen Life Sciences
IL-18BP (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) - InVivoKine™
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CHF 440.00
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AG-40B-0276-C05050 µgCHF 440.00
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Figure 1: Protein structure of IL-18BP (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) (Prod. No. AG-40B-0276).
| Product Details | |
|---|---|
| Synonyms | Interleukin-18-binding Protein (human) (monomeric):Fc (LALA-PG) Knobs-into-Holes (human) (rec.); Interferon gamma-inducing Factor-binding Protein; Tadekinig-alfa |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
Human IL-18BP (aa 31-194) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity | Human |
| Specificity |
Binds to human IL-18. Fc (LALA-PG)-KIH (human) does not bind to FcRs. |
| MW | ~70kDa and 28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg protein (LAL test). |
| Concentration | After reconstitution: 1mg/ml |
| Reconstitution | Reconstitute with 50μl endotoxin-free water. |
| Accession Number | O95998 |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control |
Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0018) |
| Other Product Data |
Uniprot link O95998: IL-18BP (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
IL-18, initially named interferon-γ inducing factor (IGIF) is an IL-1 family member and a cytokine that links innate and adaptive immunity. Depending on the microenvironment and timing, IL-18 can either drive harmful inflammation or restore immune homeostasis.
IL-18 is regulated i) by the inflammasome that converts the inactive pro-IL-18 to its active form IL-18; ii) by the cytokine it induces, the master cytokine IFN-γ that triggers the expression of the inhibitor of IL-18, called IL-18 binding protein (IL-18BP). IL-18 binding protein (IL-18BP) is one of only four natural cytokine antagonists. IL-18BP has an exceptionally high affinity for IL-18 and its slow dissociation rate makes it an effective regulator of IL-18, essential for maintaining immune balance and influencing disease outcomes.
The protein IL-18BP (human) (monomeric):Fc (LALA-PG)-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-18BP (human):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 70kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IL-18BP (human) (monomeric):Fc-KIH (human) (rec.). The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical, and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
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