Cystatin C (mouse) (monomer):Fc (silent) InVivoKine™

Specifications / Handling

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Product Details
Synonyms	Fc (LALA-PG)-KIH:Cystatin-3 (mouse)
Product Type	Protein
Properties
Source/Host	HEK 293 cells. Produced using animal component-free medium.
Sequence	The extracellular domain of mouse Cystatin C (aa 21-140) is fused to the C-terminus of the Fc (LALA-PG) Knob region of human IgG1. Fc (LALA-PG) Knobs:Cystatin C (mouse) and Fc (LALA-PG) Holes form the Cystatin C (mouse) (monomer):Fc (silent) InVivoKine™ using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).
Crossreactivity	Human Mouse
Specificity	The Fc contains the mutations LALA-PG that abolish the interaction between the Fc and FcγRs and therefore Fc undesirable effects. The Fc (LALA-PG) is named Fc (silent).
MW	~50kDa and 30 kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.0019 EU/μg purified protein (LAL test).
Concentration	1mg/ml after reconstitution.
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	P21460
Formulation	Lyophilized. Contains PBS
Protein Negative Control	Fc (silent) InVivoKine™ (human) IgG1 Control (Prod. No. AG-35B-0018)
Other Product Data	NCBI reference P21460 : Cystatin C (mouse)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Cystatin C (Cyst-C) is a member of the cystatin superfamily of cysteine protease inhibitors. Cystatin C is distributed in different organs and tissues and is associated with various immune responses to exogenous and endogenous antigens. The cystatin C gene is regulated by several factors during inflammation and infection. Cystatin C exerts its immunomodulatory functions by controlling the activity of cysteine proteases and other mechanisms.

For years, it has been observed that peripheral cancer inhibits amyloid pathology and rescues cognition in Alzheimer's disease. In a recent study, it was shown that Cystatin C secreted from peripheral tumor cells effectively reduces amyloid plaque burden and rescues cognition in mouse models of AD. Cystatin C, which acts as a ligand of triggering receptor expressed on myeloid cells 2 (TREM2), directly binds Aβ1-42 oligomers and activates TREM2 and enables microglia to phagocytose and degrade the pre-existing amyloid plaques.

The Cystatin C (mouse) (monomer):Fc (silent) InVivoKine™ is produced by using two different vectors, one encoding for the Fc Knobs (LALA-PG) (human):Cystatin C (mouse) sequence (synthesizing a protein of 50kDa) and one encoding for the Fc Holes (LALA-PG) sequence (synthesizing a protein of 30kDa). Both vectors, transfected into HEK293 cells, produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Cystatin C (mouse (monomer):Fc (silent) InVivoKine™. This Fc-KIH format allows our mouse Cystatin C protein to form a monomer Cystatin C fused to Fc, keeping its endogenous monomeric structure. The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

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