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AdipoGen Life Sciences
3-Hydroxykynurenine Transaminase (Anopheles gambiae) (rec.) (untagged)
590
CHF
CHF 590.00
In stock
AG-40T-0100-C05050 µgCHF 590.00
| Product Details | |
|---|---|
| Synonyms | 3-HKT; EC 2.6.1 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Full-length 3-HKT (Anopheles gambiae) is untagged. |
| Application | Can be used to test potential xanthurenic acid synthesis inhibitors, by performing in vitro transamination assays followed by HPLC-UV analysis (Lit. 1). |
| Biological Activity | Specific activity towards D,L-3HK: 33 μmol/min per mg. Primarily catalyses the pyridoxal 5'-phosphate (PLP)-dependent irreversible transamination of 3-hydroxykynurenine (3-HK) to xanthurenic acid (XA). |
| MW | ~44.3kDa |
| Purity | ≥99% (SDS-PAGE) |
| Endotoxin Content | N/A |
| Concentration | 0.5mg/ml (Lot specific) |
| Formulation | Liquid. In 20mM TRIS-HCl pH 8.0, containing 150mM NaCl and 40μM pyridoxal 5'-phosphate (PLP). |
| Other Product Data | UniProt link Q7PRG3: 3-HKT (Anopheles gambiae) |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice |
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The 3-hydroxykynurenine transaminase (3-HKT) gene plays a vital role in the development of malaria parasites by participating in the synthesis of xanthurenic acid, which is involved in the exflagellation of microgametocytes in the midgut of malaria vector species. The 3-HKT enzyme is involved in the tryptophan metabolism of Anophelines. The gene had been studied in the important global malaria vector, Anopheles gambiae.
Product References
- Identification and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase: F. Rossi, et al.; FEBS J. 272, 5653 (2005)
- Crystal structure of the Anopheles gambiae 3-hydroxykynurenine transaminase: F. Rossi, et al.; PNAS 103, 5711 (2006)
- Study of Anopheles gambiae 3-hydroxykynurenine transaminase activity and inhibition by LC-MS/MS method J. Pharm. Biomed. Anal. 173, 154 (2019)
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