Gasdermin E (human) ELISA Kit

CHF 690.00
In stock
AG-45B-0024-KI0196 wellsCHF 690.00
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Product Details
Synonyms GSDME; DFNA5; ICERE-1; Inversely Correlated with Estrogen Receptor Expression 1
Product Type Kit
Properties
Application Set Quantitative ELISA
Specificity

Detects human Gasdermin E (C-terminal and full-length) in serum, plasma and cell culture supernatant.

Crossreactivity Human
Quantity

1 x 96 wells

Sensitivity 50pg/ml
Range 0.0625 to 4ng/ml
Sample Type Cell Culture Supernatant
Plasma
Serum
Assay Type Sandwich
Detection Type Colorimetric
Other Product Data

UniProt link O60443: Gasdermin E (human)

Accession Number O60443
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Plate and reagents should reach room temperature before use.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Programmed cell death is a key mechanism involved in several biological processes ranging from development and homeostasis to immunity. Apoptosis, pyroptosis and NETosis are three well‐studied modes of cell death. Pyroptosis is a form of necrotic cell death that has emerged as an important innate immune mechanism against intracellular pathogens. All three modes of cell death cross‐talk between them and converge onto the activation of the same cell death effector—the pore‐forming protein Gasdermin D (GSDMD). The inflammasome platform leads to activation of caspase-1 and also the recently characterized caspase-11 (non-canonical inflammasome pathway), leading to cleavage of the intracellular protein Gasdermin D. Upon caspase-1/11 cleavage of the Gasdermin N- and C-domain linker, the cleaved N-terminal fragment of Gasdermin D oligomerizes and forms pores on the host cell membrane, leading to a cell death called pyroptosis. The Gasdermin family is an important protein that mediates pyroptosis, it can induce cell death and promote the release of inflammatory factors. It is mainly composed of six members: Gasdermin A (GSDMA), Gasdermin B (GSDMB), Gasdermin C (GSDMC), GSDMD, Gasdermin E (GSDME; also called deafness, autosomal dominant 5, DFNA5), and DFNB58. Recent studies found that GSDME features a caspase‐3 /-7 cleavage motif in its linker region. Similar to GSDMD, cleavage of GSDME by caspases‐3 /-7 liberates the N‐terminal pyroptosis‐inducing domain (GSDMENT) from its autoinhibitory C‐terminal regulatory domain to trigger membrane pores and pyroptosis. Gasdermin E is expressed in different tissues /cells such as placenta, brain, heart, kidney, cochlea, intestines, and IgE-primed mast cells. Specific cells express GSDME, and in such cells caspase-3 activation is switched from driving an apoptotic program to causing pyroptosis. Thus, certain cells are programmed to undergo pyroptosis instead of apoptosis. GSDME-knockout mice were shown to be resistant to the toxicity of chemotherapy drugs such as cisplatin. GSDME-N terminal domain also permeabilizes the mitochondrial membrane, releasing cytochrome c and activating the apoptosome. Recently it has been reported that granzyme B from killer cells activates caspase-independent pyroptosis in target cells by directly cleaving GSDME at the same site as caspase-3. Mutations in GSDME in human are associated with development of heritable, nonsyndromal deafness. GSDME expression is suppressed in many cancers and reduced GSDME is associated with decreased breast cancer survival. GSDME gene expression has been shown to be associated with favorable prognosis following chemotherapy, and it could therefore be a potential predictive biomarker.

 The Gasdermin E (human) ELISA Kit (AG-45B-0024) detects the C-terminal domain of human Gasdermin E as well as the full-length protein. Upon cleavage of Gasdermin E and pore formation, the C-terminus fragment should be released in the extracellular space and  found in serum / plasma. The role of the secreted C-terminus fragment of Gasdermin protein family has not been characterized yet.

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