DAPI . dihydrochloride

CHF 45.00
In stock
AG-CR1-3668-M0055 mgCHF 45.00
AG-CR1-3668-M02525 mgCHF 155.00
AG-CR1-3668-M100100 mgCHF 440.00
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Product Details
Synonyms 4',6-Diamidino-2-phenylindole dihydrochloride; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; FxCycle Violet
Product Type Chemical
Properties
Formula

C16H15N5 . 2HCl

MW 277.3 . 72.9
CAS 28718-90-3
RTECS NL5995050
Purity Chemicals ≥97% (HPLC)
Appearance Yellow solid.
Solubility Soluble in water (10mg/ml). May require heating and sonication. Slightly soluble in DMSO, ethanol, methanol or PBS.
Identity Determined by 1H-NMR.
Other Product Data

Note: Stock solutions (1mg/ml) should be stored in the dark and are stable in the dark at 2-8 °C for 1-2 weeks. For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.

InChi Key FPNZBYLXNYPRLR-UHFFFAOYSA-N
Smiles [H]Cl.[H]Cl.NC(C1=CC=C2C(NC(C3=CC=C(C(N)=N)C=C3)=C2)=C1)=N
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Store solutions at -20°C in the dark.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
  • Cell permeable fluorescent DNA stain. AT-sequence-specific DNA intercalator.
  • Binds to the minor groove of double-stranded DNA (preferentially to adenine and thymine (AT) rich DNA), like Hoechst dyes, forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. Spectral Data: Excitation: λex 340 nm; Emission: λem 488 nm (only DAPI). Excitation: λex 360nm; Emission: λem 460nm (DAPI-DNA complex).
  • Commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications.
  • DAPI is often used as a counterstain, as its ultraviolet excitation (λex 360nm) and blue emission (λem 460nm) wavelengths separate it nicely from many popular primary fluorophores.
  • Can be used on either fixed or live cells, although it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower and demands higher concentrations to be used.
  • Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and KAO (diamine oxidase).
Product References
  1. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses:  W.C. Russell, et al.; Nature 253, 461 (1975)
  2. DIPI and DAPI: fluorescence banding with only negliglible fading: W. Schnedl, et al.; Hum. Genet. 36, 167 (1977)
  3. An evaluation of DNA fluorochromes, staining techniques, and analysis for flow cytometry. I. Unperturbed cell populations: I.W. Taylor & B.K. Milthorpe; J. Histochem. Cytochem. 28, 1224 (1980)
  4. A comparative study of DAPI, DIPI, and Hoechst 33258 and 33342 as chromosomal DNA stains: F. Otto & K.C. Tsou; Stain Technol. 60, 7 (1985)
  5. Estimation of total DNA in crude extracts of plant leaf tissue using 4',6-diamidino-2-phenylindole (DAPI) fluorometry: L.S. Lee & H.M. Garnett; J. Biochem. Biophys. Methods 26, 249 (1993)
  6. 4',6-Diamidino 2-phenylindole is a new reversible inhibitor of diamine oxidase and S-adenosyl-L-methionine decarboxylase from mammalian tissues: C. Cubria, et al.; Comp. Biochem. Physiol. C. 105, 251 (1994)
  7. Flow cytometric detection of apoptosis: comparison of the assays of in situ DNA degradation and chromatin changes: M.A. Hotz, et al.; Cytometry 15, 237 (1994)
  8. DAPI: a DNA-specific fluorescent probe: J. Kapuscinski; Biotech. Histochem. 70, 220 (1995)
  9. The use of DAPI as a replacement for ethidium bromide in forensic DNA analysis: E. Buel & M. Schwartz; J. Forensic Sci. 40, 275 (1995)
  10. Visualizing chromatin and chromosomes in living cells: D.  Zink, et al.; Methods: A Companion to Methods in Enzymology 29, 42 (2003)
  11. Propidium Iodide (PI) or DAPI Staining of Unfixed Solid Tissues for Flow Cytometry: L. Rodgers; CSH Protoc. 2006, pdb.prot4438 (2006)
  12. DAPI Staining of Drosophila Embryos: W.F. Rothwell & W. Sullivan; CSH Protoc. 2007, pdb.prot4834 (2007)
  13. Labeling nuclear DNA using DAPI: B. Chazotte; CSH Protoc. 2011, pdb.prot5556 (2011)
  14. An investigation of the photophysical properties of minor groove bound and intercalated DAPI through quantum-mechanical and spectroscopic tools: A. Biancardi, et al.; Phys. Chem. Chem. Phys. 15, 4596 (2013)
  15. DAPI staining and fluorescence microscopy techniques for phytoplasmas: N.M. Andrade & N.L. Arismendi; Methods Mol. Biol. 938, 115 (2013)
  16. The hazards of DAPI photoconversion: effects of dye, mounting media and fixative, and how to minimize the problem: M. Jez, et al.; Histochem. Cell Biol. 139, 195 (2013)
  17. Fluorescence lifetime imaging of DAPI-stained nuclei as a novel diagnostic tool for the detection and classification of B-cell chronic lymphocytic leukemia: G. Yahav, et al.; Cytometry A 89, 644 (2016)
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