Ancell

anti-TCR Cβ1 (human), mAb (Jovi-1) (Biotin)

CHF 618.00

In stock

ANC-101-030100 µgCHF 618.00

Figure 1: 5x10^5 ficoll prepared human PBMCs were washed and pre-incubated 10 min with 20µl of 300µg/ml human IgG (to reduce non specific binding) after which they were incubated 45 min with 80µl of anti-TCR Cβ1/Biotin at 5µg/ml. Cells were washed twice and incubated with 50µl of 2nd reagent Strep/PE after which they were washed three times, fixed and analyzed by FACS. A 34% subpopulation of the cells stained positive with a mean shift of 2.09 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control (ANC-281-030). Binding was blocked when cells were pre-incubated 10 min with 20µl of 0.5mg/ml anti-TCR Cβ1 antibody (ANC-101-020).

Specifications / Handling

More Information
Product Details
Synonyms	TCRB; T Cell Receptor β Locus; TRBC1
Product Type	Monoclonal Antibody
Properties
Clone	Jovi-1 [also called Jovi.1]
Isotype	Mouse IgG2a
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Human HA1.7 TCR beta chain expressed on transgenic mouse cells.
Label/Conjugates	Biotin
Application	Flow Cytometry (1:50) FUNC: Mitogenic for T cells expressing TCR Cβ1. IHC: (see P.M. Maciocia, et al.; Nat. Med. 23, 1416 (2017))
Crossreactivity	Human
Specificity	Recognizes human Cβ1 TCR gene product (TRBC1) and reacts with 50-75% of T cells in normal human blood. Does not bind to TCR Cβ2 (TRBC2).
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein A purified.
Formulation	50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Isotype Negative Control	Mouse IgG2a Isotype Control (Biotin)
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by Ancell Corporation.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at +4°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Human T cell receptor (TCR) is a T cell surface expressed heterodimer of α/β or γ/δ chains. TCR’s recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells. This specific TRBC1 antibody has been described to be useful in a simplified immunophenotypic assessment of T cell clonality.

Product References

Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice: J.L. Viney, et al.; Hybridoma 11, 701 (1992)
T cell antigen receptor signal transduction: a tale of tails and cytoplasmic protein-tyrosine kinases: A. Weiss, et al.; Cell 73, 209 (1993)
Targeting the T cell receptor β-chain constant region for immunotherapy of T cell malignancies: P.M. Maciocia, et al.; Nat. Med. 23, 1416 (2017)
Single Antibody Detection of T-Cell Receptor αβ Clonality by Flow Cytometry Rapidly Identifies Mature T-Cell Neoplasms and Monotypic Small CD8-Positive Subsets of Uncertain Significance: M. Shi, et al.; Cytometry B Clin. Cytom. 98, 99 (2020)

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