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Ancell
anti-CD66b (human), mAb (ANC3C5) (R-PE)
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CHF 473.00
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ANC-219-050120 testsCHF 473.00
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Figure 1: Binding of anti-CD66b (human), mAb (ANC3C5) (R-PE) to human PMN.
Method: Five x 10^5 Polymorphprep prepared human Granulocytes (PMN) per tube were pelleted and pre incubated 5 minutes with 20µl of human IgG at 300µg/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 µl of anti-CD66b/R-PE (#ANC-219-050) at a 1:50 dilution (10 µg/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to a Mouse IgG1/R-PE isotype control (#ANC-278-050). Binding was blocked when cells were pre-incubated with 20 µl of 0.5 mg/ml anti-CD66b antibody (#ANC-219-020).
Method: Five x 10^5 Polymorphprep prepared human Granulocytes (PMN) per tube were pelleted and pre incubated 5 minutes with 20µl of human IgG at 300µg/ml (to reduce non specific binding) after which they were washed and incubated 45 minutes on ice with 80 µl of anti-CD66b/R-PE (#ANC-219-050) at a 1:50 dilution (10 µg/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to a Mouse IgG1/R-PE isotype control (#ANC-278-050). Binding was blocked when cells were pre-incubated with 20 µl of 0.5 mg/ml anti-CD66b antibody (#ANC-219-020).
| Product Details | |
|---|---|
| Synonyms | CEACAM8 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | ANC3C5 |
| Isotype | Mouse IgG1κ |
| Immunogen/Antigen | Recombinant soluble human CD66b. |
| Label/Conjugates | R-PE |
| Application |
ELISA |
| Crossreactivity | Human |
| Specificity |
The antibody clone ANC3C5 binds to CD66b on the cell surface of human PMN in Flow cytometry and works as an EIA capture or detection reagent for recombinant CD66b when paired with clone ANC4A4 (#ANC-220-020). |
| Purity | Protein A purified. |
| Concentration | 0.5mg/ml |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2%BSA, 0.04NaN3 |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | P31997 |
| Declaration | Manufactured by Ancell Corporation. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
Do not freeze. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Human CD66b (CEACAM8, CGMb, NCA-95, CD67) is a 95kD member of the Carcinoembryonic Antigen (CEA) group. It is expressed on granulocytes and functions as an adhesion molecule. Similar to the CD66 a, c, and d isoforms, engagement of CD66b on neutrophils causes increased adhesion to endothelial cells. Crosslinking CD66b on neutrophils with antibodies triggers IL-8 release.
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