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Ancell
anti-TIGIT (human), mAb (ANCTG6/10A6) (R-PE)
455
CHF
CHF 455.00
In stock
ANC-340-050120 testsCHF 455.00
Figure 1: Binding of anti-TIGIT/PE to human PBL. Method: Five x 10^5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated 45 minutes on ice with 20µl of 300µg/ml human IgG (to reduce non-specific binding) after which they were incubated with 80µl of anti-TIGIT/PE at a 1:50 (10µg/ml) dilution. Cells were washed three times, fixed and analyzed by FACS using a lymphoid gate. A 36% sub population of the cells stained positive with a mean shift of 0.99 log10 fluorescent units when compared to a Mouse IgG1/R-PE Isotype control (#ANC-278-050). Binding was blocked when cells were pre incubated with 20µl of 0.5 mg/ml anti-TIGIT antibody (#ANC-340-050).
| Product Details | |
|---|---|
| Synonyms | T Cell Immunoreceptor with Ig and ITIM Domains; V-set and Immunoglobulin Domain-containing Protein 9; V-set and Transmembrane Domain-containing Protein 3; Vstm3; VSIG9; WUCAM |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | ANCTG6/10A6 |
| Isotype | Mouse IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant human TIGIT. |
| Label/Conjugates | R-PE |
| Application |
ELISA Flow Cytometry |
| Crossreactivity | Human |
| Specificity |
Binds to receptors on a subset of human peripheral blood lymphoid cells, as well as to recombinant TIGIT in EIA. |
| Purity | Protein A purified. |
| Formulation | 50mM Sodium Phosphate pH 7.5, 500mM KCl, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative). |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | Q495A1 |
| Declaration | Manufactured by Ancell Corporation. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
Do not freeze. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
TIGIT (T cell immunoreceptor with Ig and ITIM domains) is a novel member of the immunoglobulin super family (IgSF). TIGIT and CD226 bind the same ligands, the two nectin-family members poliovirus receptor (PVR) and poliovirus receptor-related 2 (CD112) and compete for each other. TIGIT inhibits while CD226 activates the signaling through PVR and CD112. TIGIT and CD226 directly interact at the cell surface and this interaction impairs CD226 homodimerization and function. TIGIT is absent on naive T cells, but is expressed on activated Tregs and memory T cells, and on NK cells and NKT cells. TIGIT negatively regulates T cell activation and blockade of TIGIT exacerbates several immune diseases. TIGIT also activates the PVR receptor on dendritic cells (DCs). Addition of TIGIT (human):Fc (human) to DCs triggers phosphorylation of PVR and elicits downstream signaling, which then inhibits T cell responses by producing IL-10. TIGIT also negatively regulates NK cell cytotoxicity and cytokine production. TIGIT is directly regulated by FoxP3 and promotes inducible Treg differentiation.
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