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BellBrook
eIF4A1 (human) (rec.) (His) (active) (25µg)
As low as
530
CHF
CHF 530.00
In stock
Only %1 left
BBL-2383-C02525 µgCHF 530.00
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Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Eukaryotic Initiation Factor 4A-I; DDX2A; ATP-dependent RNA Helicase eIF4A-1 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Full-length human eIF4A1 protein with an N-terminal 6x His-tag. |
| Crossreactivity | Human |
| Application |
For use with Transcreener ADP2 assay. |
| Biological Activity |
The enzyme has been thoroughly validated with Transcreener ADP² Activity Assays. For specific activity please refer to the Certificate of Analysis for each individual enzyme lot. |
| MW | ~47kDa |
| Purity | ≥90% (SDS-PAGE) |
| Accession Number | P60842 |
| Formulation | Liquid. 50mM Tris-HCl, 500mM NaCl, 10% Glycerol (pH 8). |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer Uniprot P60842: EIF4A1_HUMAN |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Scientific Background Information
Product Description
eIF4A1 is an ATP-dependent RNA helicase of the DEAD-box protein family and an essential component of the eukaryotic translation initiation machinery. As part of the eIF4F complex, together with eIF4E and eIF4G, eIF4A1 facilitates ribosome recruitment and scanning on mRNA. eIF4A1 binds RNA and ATP and uses ATP hydrolysis to remodel and unwind RNA secondary structures, particularly within 5′ untranslated regions (5′ UTRs). This activity supports efficient translation initiation and is especially important for mRNAs with highly structured 5′ UTRs. eIF4A1 is also studied as a regulator of selective mRNA translation and has been linked to cellular proliferation, stress responses and cancer biology. Recombinant eIF4A1 is suitable for studies of RNA helicase and ATPase activity, translation initiation, protein-RNA interactions and screening of eIF4A1 modulators.
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