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BellBrook
Transcreener UDP2 FI Assay
CHF 0.00
In stock
BBL-3019-A200 assaysCHF 532.00
BBL-3019-1K1000 assaysCHF 1'011.00
BBL-3019-10K10000 assaysCHF 5'152.00
BBL-3019-100K10 x 10000 assaysINQ
Figure 1: Schematic overview of the Transcreener UDP2 FI Assay.
The Transcreener UDP Detection Mixture contains a quenched UDP Alexa Fluor 594 tracer bound to an UDP2 antibody conjugated to an IRDye QC-1 quencher. UDP produced by the target enzyme displaces the tracer, which is no longer quenched and causes a positive increase in FI.
The Transcreener UDP Detection Mixture contains a quenched UDP Alexa Fluor 594 tracer bound to an UDP2 antibody conjugated to an IRDye QC-1 quencher. UDP produced by the target enzyme displaces the tracer, which is no longer quenched and causes a positive increase in FI.
| Product Details | |
|---|---|
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | All |
| Quantity |
200 assays/96wells (BBL-3019-A) 1000 assays/384wells (BBL-3019-1K) 10000 assays/384wells (BBL-3019-10K) 10 x 10000 assays/384wells (BBL-3019-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Sensitivity | Excellent data quality (Z’ ≥ 0.7) at low substrate conversion using 1µM UDP. |
| Range | Accommodates UDP concentrations ranging from 1 μM to 100 μM. |
| Detection Type | Fluorescence intensity |
| Kit Contains |
UDP2 Antibody-IRDye® QC-1, UDP Alexa Fluor® 594 Tracer, Stop & Detect Buffer B, 10X, UDP Note: The plates are not included, please see manual for material not provided but necessary for assay. |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer
Easy-to-Use (mix-and-read method).Simple homogenous biochemical assay. Direct detection of UDP eliminates laborious coupling steps. HTS Compatible with 96, 384, and 1536-well plates. Outstanding deck and signal stability. Excellent sensitivity. |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.
The Transcreener UDP2 assay is available in three fluorescent readouts, Fluorescence polarization (FP), Fluorescence Intensity (FI) and Time-resolved Forster-resonance-energy-transfer (TR-FRET), which provide a safe HTS-compatible alternative to cumbersome radioassay methods and are more sensitive and less subject to interference than other detection methods.
The Transcreener® UDP2 FI Assay (Prod. No. BBL-3019) is a red, competitive fluorescence intensity (FI) assay. Because it is highly selective for UDP, the assay can be used with any enzyme that produces UDP, regardless of what other substrates are used. Examples include glycosyltransferases, galactosyltransferases, glucuronyltransferases, N-acetylglucosamyltransferases, N-acetylgalactosyltransferases, xylosyltransferases, and glycogen, cellulose, lactose, and hyaluronan synthases. The Transcreener® UDP2 Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to UDP concentrations (1 to 100 μM). The assay provides excellent signal at low substrate conversion, with a Z’ ≥ 0.7 at low substrate conversion.
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