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BellBrook
Transcreener CD73 FP Assay
CHF 0.00
In stock
BBL-3026-1K1000 assaysCHF 1'234.00
BBL-3026-10K10000 assaysCHF 6'497.00
BBL-3026-100K10 x 10000 assaysINQ
Figure 1: Schematic overview of Transcreener CD73 FP Assay.
| Product Details | |
|---|---|
| Synonyms | Ecto-5'-nucleotidase |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | All |
| Quantity |
1000 assays/384wells (BBL-3026-1K) 10000 assays/384wells (BBL-3026-10K) 10 x 10000 assays/384wells (BBL-3026-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Range | Excellent data quality (Z’ ≥ 0.7) and signal (≥85 mP polarization shift) at ADO ranges between 0.3 μM and 3 μM. |
| Detection Type | Fluorescent polarization |
| Kit Contains |
ADP2 Antibody, ADP Alexa Fluor® 633 Tracer, Coupling Enzyme, ATP, AMP, ADO. Note: The plates are not included, please see manual for material not provided but necessary for assay. |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer
Detection of unlabeled Adenosine |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Manual |
 Download PDF |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.
The Transcreener CD73 Assay measures adenosine (ADO) produced by ecto-5'-nucleotidase (also known as 5'-nucleotidase, NT5E, Cluster of Differentiation 73 or CD73). By measuring the production of ADO researchers can effectively determine the activity of the enzyme. The assay provides a powerful tool to screen entire compound libraries for CD73 modulators to help find new therapeutics for diseases such as cancer.
The assay uses a coupling enzyme that uses ADO produced by CD73 to produce the products AMP and ADP. ADP can then be measured using the trusted Transcreener ADP² Assay components. The kit comes complete with all the detection reagents required to measure activity. CD73 enzyme is not included in the inhibitor screening assay kit.
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