Transcreener dAMP Exonuclease FP Activity Assay

The Transcreener^® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener^® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.

The Transcreener® dAMP FP Assay is a far-red, competitive fluorescence polarization (FP) assay. The assay is designed to be used with exonuclease enzymes such as TREX1 (also known as Three Prime Repair Exonuclease 1), that produce the product dAMP (deoxyadenosine monophosphate, deoxyadenylic acid, or deoxyadenylate) by cleaving sources of DNA. An example is the human enzyme TREX1 which is the major exonuclease responsible for degrading cytosolic DNA.

The Transcreener assay is designed specifically for high throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The Transcreener dAMP FP Assay provides a simple single addition exonuclease activity assay capable of HTS. Excellent data quality (Z’ ≥ 0.7) and signal (≥85 mP polarization shift) at dAMP ranges between 0.1-10 μM. Sensitive detection of dAMP down to 10 nM and as high as 100 μM.