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BellBrook
Transcreener OAADPr SIRT FP Assay
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BBL-3046-1K1000 assaysCHF 1’025.00
BBL-3046-10K10000 assaysCHF 5’699.00
BBL-3046-100K10 x 10000 assaysINQ
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Figure 1: Schematic Overview of the Transcreener OAADPr SIRT FP Assay. OAADPr produced by the target SIRT enzyme is completely converted to AMP in real time by the OAADPr Coupling Enzyme. In the detection step, the CE reaction is quenched by EDTA, and AMP displaces an Alexa Fluor® 633 Tracer from the AMP2/GMP2 Antibody, resulting in decreased fluorescence polarization.
| Product Details | |
|---|---|
| Synonyms | 2’-O-acetyl-ADP-ribose; Sirtuin |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | All |
| Quantity |
1000 assays/384wells (BBL-3046-1K) 10000 assays/384wells (BBL-3046-10K) 100000 assays/384wells (BBL-3046-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Sensitivity | Excellent data quality (Z’ ≥ 0.7) |
| Detection Type | Fluorescent polarization |
| Kit Contains |
AMP2/GMP2 Antibody Note: The plates are not included. An entirely black plate with a non-binding surface is required. We suggest Corning 384-well plates #4514; sold under #BBL-2229 or BBL-2230). |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer Assay Features It is easy to integrate into automated HTS workflows, with outstanding reagent stability (deck stability > 16 hours, signal stability > 24 hours), robust detection of OAADPr, and compatibility with commonly used multimode plate readers. Data quality is excellent (Z’ ≥ 0.7), and the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering. |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.
The Transcreener OAADPr SIRT FP Assay is a biochemical HTS assay for measuring the production of 2’-O-acetyl-ADP-ribose (OAADPr) by sirtuins (SIRT), NAD-dependent deacetylases. The assay relies on a Coupling Enzyme (CE) to convert OAADPr into AMP, which is then detected using a far-red, competitive fluorescence polarization (FP) assay. As an example, the Transcreener OAADPr SIRT FP assay can be used to detect the activity of human SIRT1 and SIRT2 using acetylated peptides as substrates.
The Transcreener OAADPr SIRT Assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It is easy to integrate into automated HTS workflows, with outstanding reagent stability (deck stability > 16 hours, signal stability > 24 hours), robust detection of OAADPr, and compatibility with commonly used multimode plate readers. Data quality is excellent (Z’ ≥ 0.7), and the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.