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BellBrook
Enzolution™ SIRT2 FP Assay System
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BBL-3049-FP-1K1000 assaysCHF 683.00
BBL-3049-FP-10K10000 assaysCHF 1’925.00
BBL-3049-FP-100K10 x 10000 assaysINQ
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Figure 1: Schematic overview of the Enzolution™ SIRT2 FP Assay System with the Transcreener OAADPr SIRT Assay.
| Product Details | |
|---|---|
| Synonyms | SIRT2; NAD-dependent Deacetylase Sirtuin-2; SIR2-like protein 2; SIR2L2 |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | Human |
| Quantity |
1'000 assays/384wells (BBL-3049-FP-1K) 10'000 assays/384wells (BBL-3049-FP-10K) 100'000 assays/384wells (BBL-3049-FP-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Detection Type | Fluorescent polarization |
| Kit Contains |
- SIRT2 (human) (rec.) Enyzme |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer |
| Declaration | Manufactured by BellBrook Labs. |
| Accession Number | P09874 |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Enzolution SIRT2 Assay System is intended for use with the Transcreener OAADPr SIRT Assay Kits (Parts #3046 and #3047) to measure the enzymatic activity of SIRT2 (NAD-dependent protein deacetylase sirtuin 1). SIRT2 catalyzes transfer of acetyl groups from lysine residues to NAD, yielding the unacetylated peptide, O-acetyl-ADP ribose (OAADPr), and nicotinamide as products. The assay relies on Coupling Enzymes (CE) to convert OAADPr into AMP, which is then detected using a far-red, competitive fluorescence polarization (FP) or time-resolved Förster-resonance-energy-transfer (TR-FRET) assay. The assay has been optimized and extensively validated for high-throughput screening (HTS) and inhibitor dose response measurements using most multimode plate readers.
The Enzolution SIRT2 Assay System provides all reagents required to screen and profile SIRT2 inhibitors when used with the Transcreener OAADPr SIRT Assay Kits, including purified, full length human SIRT2 (aa 1-389) and acetylated peptide, H3K9Ac (1-21). The protocol is configured for 384-well plates; use of different multi-well plate formats will require adjustment of reagent concentrations utilized in the assay.
When purchasing this Enzolution Assay System, it is important to choose the assay system readout that corresponds to the FP configuration of your Transcreener pADPr Assay.
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