BellBrook

Transcreener OAADPr SIRT TR-FRET Assay

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BBL-3047-1K1000 assaysCHF 1’139.00

BBL-3047-10K10000 assaysCHF 6’269.00

BBL-3047-100K10 x 10000 assaysINQ

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Figure 1: Schematic Overview of the Transcreener OAADPr SIRT TR-FRET Assay. OAADPr produced by the target SIRT Enzyme is completely converted to AMP in real time by the OAADPr Coupling Enzyme. In the detection step, the CE reaction is quenched by EDTA, and AMP displaces a Hilyte 647 Tracer from an AMP2/GMP2 Antibody conjugated to Terbium (Tb), resulting in a decrease in TR-FRET.

Specifications / Handling

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Product Details
Synonyms	2’-O-acetyl-ADP-ribose; Sirtuin
Product Type	Kit
Properties
Application Set	Compound Screening
Crossreactivity	All
Quantity	1'000 assays/384wells (BBL-3047-1K) 10'000 assays/384wells (BBL-3047-10K) 100'000 assays/384wells (BBL-3047-100K) Note: The exact number of assays depends on enzyme reaction conditions.
Sensitivity	Excellent data quality (Z’ ≥ 0.7)
Detection Type	TR-FRET
Kit Contains	AMP2/GMP2 Antibody-Tb, 800nM AMP2/GMP2 Hilyte 647 Tracer, 10µM OAADPr Coupling Enzyme (200X) NAD+, 5mM AMP, 5 mM Stop & Detect Buffer B, 10X Enzyme Assay Buffer G, 10X DTT, 1M MnCl₂, 1M Note: Note: The plates are not included. An entirely white plate with a non-binding surface is required. We suggest Corning 384-well plates #4513; sold under #BBL-2231 or BBL-2232).
Other Product Data	Click here for Complete Information from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. Assay Features It is easy to integrate into automated HTS workflows, with outstanding reagent stability (deck stability > 16 hours, signal stability > 24 hours), robust detection of OAADPr, and compatibility with commonly used multimode plate readers. Data quality is excellent (Z’ ≥ 0.7), and the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
Declaration	Manufactured by BellBrook Labs.
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-20°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles.
Documents
Manual	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

The Transcreener^® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener^® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families. Transcreener assays have been validated with major HTS instruments to determine the optimal filters and settings for maximal assay performance. Application notes with all important details are available.

The Transcreener OAADPr SIRT TR-FRET Assay is a biochemical HTS assay for measuring the production 2’-O-Acetyl-ADP-ribose (OAADPr) by sirtuins (SIRT), NAD-dependent deacetylases. The assay relies on Coupling Enzymes (CE) to convert OAADPr into AMP, which is then detected using a far-red, competitive, time-resolved Förster-resonance-energy-transfer (TR-FRET) assay. As an example, the Transcreener OAADPr SIRT TR-FRET assay can be used to detect the activity of human SIRT1 and SIRT2 using acetylated peptides as substrates.

The Transcreener OAADPr SIRT Assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It is easy to integrate into automated HTS workflows, with outstanding reagent stability (deck stability > 16 hours, signal stability > 24 hours), robust detection of OAADPr, and compatibility with commonly used multimode plate readers. Data quality is excellent (Z’ ≥ 0.7), and the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.

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