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BellBrook
Heliscreener™ BLM Helicase DNA Unwinding Assay
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BBL-3053-1K1000 assaysCHF 909.00
BBL-3053-10K10000 assaysCHF 4’308.00
BBL-3053-100K10 x 10000 assaysINQ
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| Product Details | |
|---|---|
| Synonyms | Bloom syndrome protein; RecQ protein-like 3; RecQ-like DNA helicase BLM |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | Human |
| Quantity |
1000 assays/384wells (BBL-3053-1K) 10000 assays/384wells (BBL-3053-10K) 100000 assays/384wells (BBL-3053-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Detection Type | Fluorescent |
| Kit Contains |
- Reporter DNA, 4µM |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer |
| Declaration | Manufactured by BellBrook Labs. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Heliscreener DNA Unwinding Assay is a biochemical assay designed to measure the DNA unwinding activity of DNA helicases. The assay utilizes a labeled double-stranded DNA (dsDNA) fork substrate that is quenched when intact and emits far-red fluorescence following unwinding (see Figure 1). The assay can be performed in kinetic (continuous monitoring) or endpoint mode. Kinetic mode is recommended for initial velocity measurements, allowing for accurate enzyme kinetics and inhibitor IC50 determinations. Alternatively, for screening, the enzyme reaction can be stopped by adding the Stop & Detect Buffer B, and the quenched reactions can be read in endpoint mode. This assay is designed to be used with purified enzyme preparations. Heliscreener assays are not validated with cell lysates, blood, serum, or other biological samples.
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