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BellBrook
Enzolution eIF4A1 ATPase FI Assay System
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BBL-3055-FI-1K1000 assaysCHF 852.00
BBL-3055-FI-10K10000 assaysCHF 4’966.00
BBL-3055-FI-100K10 x 10000 assaysINQ
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Figure 1: Schematic overview of the Enzolution™ eIF4A1 ATPase Assay System with the Transcreener ADP2 Assay.
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Eukaryotic Initiation Factor 4A-I; DDX2A; ATP-dependent RNA Helicase eIF4A-1 |
| Product Type | Kit |
| Properties | |
| Application Set | Compound Screening |
| Crossreactivity | Human |
| Quantity |
1000 assays/384wells (BBL-3055-FI-1K) 10000 assays/384wells (BBL-3055-FI-10K) 100000 assays/384wells (BBL-3055-FI-100K) Note: The exact number of assays depends on enzyme reaction conditions. |
| Detection Type | Fluorescence intensity |
| Kit Contains |
- eIF4A1 (human) (rec.) (His) Enzyme - Yeast RNA (10mg/μl) - Enzyme Assay Buffer E, 10X - TCEP, 0.5M - 384-Well Low Volume Assay Plates (Corning#4514; sold under #BBL-2229 or BBL-2230) |
| Other Product Data |
Click here for Complete Information from the Original Manufacturer |
| Declaration | Manufactured by BellBrook Labs. |
| Accession Number | P60842 |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Documents | |
| Manual |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
The Enzolution EIF4A1 ATPase Assay System is intended for use with the Transcreener® ADP2 Assay Kits to measure the RNA-dependent ATPase activity of EIF4A1 (eukaryotic initiation factor 4A-I), a member of the DEAD-box family of RNA helicases (DDX2A). EIF4A1 functions as a core component of the EIF4F translation initiation complex in eukaryotic cells and is upregulated in diverse cancers. ADP formation by EIF4A1 can be easily measured with the Transcreener ADP2 assay, a far-red, competitive fluorescence assay that enables single addition, mix-and-read detection in a continuous or endpoint format. The assay has been optimized and extensively validated for high-throughput screening (HTS) and inhibitor dose-response measurements using most multimode plate readers.
The Enzolution EIF4A1 ATPase Assay System provides all reagents required to screen and profile EIF4A1 inhibitors, including purified, full-length human EIF4A1 and yeast RNA, when used with the Transcreener ADP2 Assay Kits, which are available with FP, FI and TR-FRET readouts. The protocol is configured for 384-well plates; use of different multi-well plate formats will require adjustment of reagent concentrations utilized in the assay.
When purchasing the eIF4A1 ATPase FI Assay System, it is important to choose the assay system readout corresponding to the FI configuration of your Transcreener ADP2 Assay Kit!
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