CHF 54.00
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CDX-B0148-G0011 gCHF 54.00
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CDX-B0148-G02525 gCHF 611.00
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Product Details
Synonyms Bios II; Biotin; Coenzyme R; Vitamin B7; Vitamin H; NSC 63865; Factor S
Product Type Chemical
Formula C10H16N2O3S
MW 244.3
CAS 58-85-5
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (NMR)
Appearance White to off-white powder.
Solubility Soluble in hot water or DMSO (49mg/ml at 25°C).
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

D-(+)-Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). D-(+)-Biotin is a cofactor responsible for carbon dioxide transfer in five carboxylase enzymes, such as acetyl-CoA carboxylase α, acetyl-CoA carboxylase β, methylcrotonyl-CoA carboxylase, propionyl-CoA carboxylase or pyruvate carboxylase. It is important in fatty acid synthesis, branched-chain amino acid catabolism and gluconeogenesis. It covalently attaches to the epsilon-amino group of specific lysine residues in these carboxylases. This biotinylation reaction requires ATP and is catalyzed by holocarboxylase synthetase. Biotin is also covalently attached to distinct lysine residues in histones, affecting chromatin structure and mediating gene regulation. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size means the biological activity of the protein will most likely be unaffected. This process is called biotinylation. Because both streptavidin and avidin bind biotin with high affinity (Kd of 10-14 mol/l to 10-15 mol/l) and specificity, biotinylated proteins of interest can be isolated from a sample by exploiting this highly stable interaction. The sample is incubated with streptavidin/avidin beads, allowing capture of the biotinylated protein of interest. The attachment of biotin to various chemical sites is used to study various processes, including protein localization, protein interactions, DNA transcription and replication.

Product References

(1) A. Chapman-Smith & J.E. Cronan Jr.; J. Nutr. 129, 477S (1999) | (2) J. Zempleni & D.M. Mock; J. Nutr. Biochem. 10, 128 (1999) | (3) A. Holmberg, et al.; Electrophoresis 26, 501 (2005) | (4) J. Zempleni, et al.; Biofactors 35, 36 (2009)

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