Biotinyl tyramide

CHF 161.00
In stock
CDX-B0270-M100100 mgCHF 161.00
CDX-B0270-M500500 mgCHF 643.00
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Product Details
Synonyms Biotin-Phenol; BP; N-(4-Hydroxyphenethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Product Type Chemical
Properties
Formula C18H25N3O3S
MW 363.5
CAS 41994-02-9
Source/Host Chemicals Synthetic.
Purity Chemicals ≥97% (HPLC)
Appearance White to light pink solid.
Solubility Soluble in DMSO or acetonitrile.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key VZWXNOBHWODXCW-ZOBUZTSGSA-N
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Biotin derivative. Substrate of the horseradish peroxidase enzyme and used as a reagent to amplify immunohistochemical signals. It is based on the HRP-catalyzed deposition of tyramide conjugates (such as biotinyl-tyramide) on a solid phase. Subsequent reaction with streptavidin fluorophore results in the localization of the fluorophore at the site of tyramide deposition. This fluorescence-based tyramide signal amplification (TSA) has been widely used in immunohistochemistry, immunoelectron microscopy, fluorescent in situ hybridization (FISH) and fluorescence ELISA. The TSA method has been reported to increase the detection sensitivity up to 100-fold as compared with conventional avidin–biotinylated enzyme complex procedures. It can be used together with both chromogenic and fluorescence visualization methods. It can be added to any other standard IHC protocol and reduces the use of other reagents; improves signal to noise by reducing the titer of other reagents in the assay protocol and enables multi-target detection in both IHC and (F)ISH applications.

Product References

(1) B. Hunyady, et al.; J. Histochem. Cytochem. 44, 1353 (1996) | (2) G. Mayer, et al.; J. Histochem. Cytochem. 45, 1449 (1997) | (3) J.A. McKay, et al.; Mol. Path. 50, 322 (1997) | (4) M.F. Evans, et al.; Mod. Pathol. 15, 1339 (2002) | (5) Q. Chenac, et al.; Anal. Lett. 45, 219 (2012) | (6) H. Gong, et al.; Anal. Bioch. 426, 27 (2012) | (7) E. Draberova, et al.; J. Immunol. Methods 395, 63 (2013)

RANKL deletion in periodontal ligament and bone lining cells blocks orthodontic tooth movement: C.Y. Yang, et al.; Int. J. Oral Sci. 10, 3 (2018)

Mapping the mammalian ribosome quality control complex interactome using proximity labeling approaches: N. Zuzow, et al.; MBoC, ahead of print (2018)

Identification of novel dense-granule proteins in Toxoplasma gondii by two proximity-based biotinylation approaches: M. Pan, et al.; J. Proteom. Res., in press (2018)

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