5-ROX

Specifications / Handling

More Information
Product Details
Synonyms	5-Carboxy-X-rhodamine; 5-CXR
Product Type	Chemical
Properties
Formula	C₃₃H₃₀N₂O₅
MW	534.6
CAS	216699-35-3
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥95% (HPLC)
Appearance	Dark purple solid.
Solubility	Soluble in methanol, DMSO or DMF.
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	UNGMOMJDNDFGJG-UHFFFAOYSA-N
Smiles	OC(=O)C1=CC=C(C(=C1)C([O-])=O)C1=C2C=C3CCC[N+]4=C3C(CCC4)=C2OC2=C3CCCN4CCCC(C=C12)=C34
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	+4°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at +4°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

5-ROX is the purified isomer of 5(6)-ROX. It is preferred for some complicated biological applications where reproducibility is really critical. The minor positional difference between 5-ROX and 6-ROX might significantly affect some biological properties of the underlying conjugates. Used for oligonucleotide labeling and automated DNA sequencing applications. ROX is a passive fluorescent dye used in real-time PCR (qPCR) for signal normalisation. By normalising the fluorescent signal, this can reduce the variability between technical replicates.

Product References

(1) P.J. Finn et al.; Nucleic Acids Res. 30, 2877 (2002) | (2) J.M. Bartlett et al.; Mol Biotechnol 13, 185 (1999) | (3) S.C. Hung et al.; Anal Biochem 255, 32 (1998)

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