5(6)-FAM N-succinimidyl ester

CHF 121.00
In stock
CDX-C0011-M100100 mgCHF 121.00
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Product Details
Synonyms 5(6)-Carboxyfluorescein NHS ester (mixture of isomers); 5(6)-CFSE; 5(6)-FAM SE; FLUOS
Product Type Chemical
Properties
Formula C25H15NO9
MW 473.4
CAS 117548-22-8
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (HPLC)
Appearance Yellow to orange powder.
Solubility Soluble in DMSO or dimethylformamide.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key STUDUJDWFUAOEN-UHFFFAOYSA-N
Smiles OC1=CC=C2C(OC(C3=C2C4=C(C(O)=O)C=CC=C4)=CC(C=C3)=O)=C1.CC(ON5C(CCC5=O)=O)=O
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

Fluorescent probe and cell staining dye. Reacts with primary and secondary amine groups on proteins and other biomolecules. Cell permeable and covalently couples, via its succinimidyl group, to intracellular molecules. Due to this covalent coupling reaction fluorescent CFSE can be retained within cells for extremely long periods. Used for immunological studies to monitor the proliferation of many cell types (including lymphocytes, fibroblasts) and even bacteria. Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA). Favored over FITC in bioconjugations, since FAM reagents are more resistant to hydrolysis. In addition, FAM reagents require less stringent conjugation conditions and give better conjugation yields, and the resulted conjugates have superior stability. Spectral properties: λex= 492; λem=517nm in DMF or 0.1 M Tris, pH 8.0.

Product References

(1) P.R. Banks & D.M. Paquette; Bioconjug. Chem. 6, 447 (1995) | (2) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996) | (3) D. Fulcher & S. Wong; Immunol. Cell Biol. 77, 559 (1999) | (4) T. Sathiyaseelan & C.L. Baldwin; Res. Vet. Sci. 69, 275 (2000) | (5) I. Tsuge, et al.; Allergol. Int. 56, 149 (2007)

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