5-FAM N-succinimidyl ester

CHF 198.00
In stock
CDX-C0015-M01010 mgCHF 198.00
CDX-C0015-M02525 mgCHF 396.00
CDX-C0015-M100100 mgCHF 1'187.00
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Product Details
Synonyms 5-Carboxyfluorescein NHS ester; 5-CFSE; 5-FAM, SE
Product Type Chemical
Properties
Formula C25H15NO9
MW 473.39
CAS 92557-80-7
Source/Host Chemicals Synthetic
Purity Chemicals ≥98% (HPLC)
Appearance Light yellow to orange powder.
Solubility Soluble in DMSO, methanol or dimethylformamide.
Identity Determined by NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key TWOKNYRPKCLMAR-UHFFFAOYSA-N
Smiles OC(=O)C1=C(C=CC(=C1)C(=O)ON1C(=O)CCC1=O)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Keep cool and dry.
Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Popular green fluorescent cell-permeable dye. Covalently couples via its succinimidyl group to intracellular molecules. The amine-reactive succinimidyl ester of 5-FAM acid is a common fluorescent derivatization reagent for covalently labeling peptides, proteins and nucleotides. Due to this covalent coupling reaction it can be retained within cells for extremely long periods and due to this stable linkage, once incorporated within cells the dye is not transferred to adjacent cells. Yields carboxyamides which are more resistant to hydrolysis than fluorescein conjugates prepared with FITC. Better retained in cells than fluorescein. Used in fluorescent small molecule preparations. Spectral data: λex 494 nm| λem 520 nm (Buffer pH 9.0).

Product References

(1) P. Breeuwer, et al.; Appl. Environ. Microbiol. 62, 178 (1996) | (2) M. Adamczyk, et al.; Bioconjugate Chem. 8, 253 (1997) | (3) S. K. Lau, et al.; J. Chromatogr. A. 809, 203 (1998) | (4) D.A. Fulcher & S.W. J. Wong; Immunol. Cell Biol. 77, 559 (1999) | (5) S. Nampalli, et al.; Bioconjugate Chem. 13, 468 (2002) | (6) L. Milroy, et al.; JACS 134, 8480 (2012)

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