Carboxy-DCFDA

CHF 122.00
In stock
CDX-C0042-M100100 mgCHF 122.00
CDX-C0042-G0011 gCHF 702.00
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Product Details
Synonyms 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate
Product Type Chemical
Properties
Formula C25H14Cl2O9
MW 529.28
CAS 127770-45-0
Source/Host Chemicals Synthetic.
Purity Chemicals ≥85% (HPCE)
Appearance White to off-white powder.
Solubility Soluble in DMSO, DMF or methanol.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Smiles CC(O)=O.CC(=O)OC1=CC2=C(C=C1Cl)C1(OC(=O)C3=C1C=CC=C3)C1=CC(Cl)=C(OC(C)=O)C=C1O2
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

Non-activated, amine-reactive fluorescent probe. Mixture of 5/6-isomers of CDCFDA that can be used in biomolecules conjugation. 5(6)-CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. 5(6)-CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. 5(6)-CDCFDA does not stain dead cells. 5(6)-CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: λex=504nm, λem=529nm.

Product References

(1) J.P. Kehrer & T. Paraidathathu; Free Rad. Res. Commun. 16, 217 (1992) | (2) Q.P. Lloyd, et al.; J. Biol. Chem. 270, 22445 (1995) | (3) A.L. Inselman, et al.; Curr. Microbiol. 38, 295 (1999) | (4) M.J. Zamek-Gliszczynski, et al.; J. Pharmacol. Exp. Therap. 304, 801 (2003) | (5) L. Li, et al.; Free Radic. Res. 42, 354 (2008) | (6) M. Medhora, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008) | (7) S. Siissalo, et al.; Eur. J. Pharm. Biopharm. 71, 332 (2009) | (8) A.I. Pogue, et al.; Int. J. Mol. Sci. 13, 9615 (2012) | (9) J. Roh, et al.; Redox Biol. 11, 254 (2016) | J.L. Roh, et al.; Cancer Lett. 381, 96 (2016)

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