|Synonyms||2',7'-Dichlorofluorescein diacetate; Diacetyldichlorofluorescein; DCFH-DA|
|Purity Chemicals||≥95% (HPLC)|
|Appearance||White to off-white powder.|
|Solubility||Soluble in DMSO or acetone.|
|Identity||Determined by NMR.|
|Declaration||Manufactured by Chemodex.|
|Other Product Data||
Click here for Original Manufacturer Product Datasheet
|Shipping and Handling|
|Short Term Storage||+20°C|
|Long Term Storage||+4°C|
Keep cool and dry.
Protect from light and moisture.
|Use/Stability||Stable for at least 2 years after receipt when stored at +4°C.|
|Product Specification Sheet|
A cell-permeable fluorogenic dye useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the degree of overall oxidative stress. The fluorescence of DCFDA indicates oxidation by hydrogen peroxide, peroxynitrite or hydroxyl radical. Superoxide anions can also contribute to DCFDA oxidation albeit at a lesser degree. It measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity within the cell. After diffusion in to the cell, DCFDA is deacetylated by cellular esterases to a non-fluorescent compound, which is later oxidized by ROS into the highly fluorescent 2’,7’-dichlorofluorescein (DCF). Spectral data: λex=504nm, λem=524nm in 0.1 M Tris pH 8.0 (esterase).
(1) A.R. Rosenkranz, et al.; J. Immunol. Meth. 156, 39 (1992) | (2) T. Takanashi, et al.; Invest. Ophthalmol. Vis. Sci. 38, 2721 (1997) | (3) E. Eruslanov & S. Kusmartsev; Methods Mol. Biol. 594, 57 (2010)