5-Carboxy-DCFDA

Specifications / Handling

More Information
Product Details
Synonyms	5-CDCFDA; 5-Carboxy-2',7'-dichlorofluorescein diacetate; 5-Carboxy-2',7'-dichlorofluorescein DA
Product Type	Chemical
Properties
Formula	C₂₅H₁₄Cl₂O₉
MW	529.28
CAS	144489-09-8
Source/Host Chemicals	Synthetic.
Purity Chemicals	≥95% (NMR)
Appearance	Off-white powder.
Solubility	Soluble in DMSO, DMF, acetonitrile, methanol or chloroform.
Identity	Determined by ¹H-NMR.
Declaration	Manufactured by Chemodex.
Other Product Data	Click here for Original Manufacturer Product Datasheet Our product description may differ slightly from the original manufacturers product datasheet.
InChi Key	AJIVVDSJIOOZOJ-UHFFFAOYSA-N
Smiles	ClC1=C(OC(C)=O)C=C2C(C3(C(C=CC(C(O)=O)=C4)=C4C(O3)=O)C(C=C(Cl)C(OC(C)=O)=C5)=C5O2)=C1
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Keep cool and dry. Protect from light and moisture.
Use/Stability	Stable for at least 2 years after receipt when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Non-activated, amine-reactive fluorescent probe. Single 5-isomer of CDCFDA that can be used in biomolecules conjugation. CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in viable cells with intact cell membranes. CFCFDA does not stain dead cells. CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells and intracellular pH levels. Spectral data: λex=504nm, λem=529nm.

Product References

(1) C.K. Raymond et al.; J. Cell Biol. 111, 877 (1990) | (2) J.P. Kehrer & T. Paraidathathu; Free Rad. Res. Commun. 16, 217 (1992) | (3) Q.P. Lloyd, et al.; J. Biol. Chem. 270, 22445 (1995) | (4) A.L. Inselman, et al.; Curr. Microbiol. 38, 295 (1999) | (5) M.J. Zamek-Gliszczynski, et al.; J. Pharmacol. Exp. Therap. 304, 801 (2003) | (6) L. Li, et al.; Free Radic. Res. 42, 354 (2008) | (7) M. Medhora, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 294, L902 (2008) | (8) S. Siissalo, et al.; Eur. J. Pharm. Biopharm. 71, 332 (2009) | (9) A.I. Pogue, et al.; Int. J. Mol. Sci. 13, 9615 (2012) | (10) J. Roh, et al.; Redox Biol. 11, 254 (2016) | (11) J.L. Roh, et al.; Cancer Lett. 381, 96 (2016)

Call	+41 61 926 6040
Fax	+41 61 926 6049