DAN

CHF 156.00
In stock
CDX-D0062-G0011 gCHF 156.00
CDX-D0062-G0055 gCHF 530.00
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Product Details
Synonyms 2,3-Diaminonaphthalene
Product Type Chemical
Properties
Formula C10H10N2
MW 158.2
CAS 771-97-1
Source/Host Chemicals Synthetic.
Purity Chemicals ≥98% (HPLC)
Appearance Off-white to light brown solid.
Solubility Soluble in DMSO, DMF or pyridine.
Identity Determined by 1H-NMR.
Declaration Manufactured by Chemodex.
Other Product Data

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Our product description may differ slightly from the original manufacturers product datasheet.

InChi Key XTBLDMQMUSHDEN-UHFFFAOYSA-N
Smiles NC1=CC2=CC=CC=C2C=C1N
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Protect from light and moisture.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.

Product References

(1) J. Pedro et al.; Anal. Chim. Acta 516(1-2), 229 (2004) | (2) T. Nagano et al.; Biol. Pharm. Bull. 21(12), 1247 (1998) | (3) W.C. Hawkes et al.; Anal. Biochem. 241(2), 206 (1996) | (4) R.F. Bayfield et al.; Anal. Biochem. 144(2), 569 (1995) | (5) P. Damiani et al.; Talanta 33(8), 649 (1986)

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