IL-2 (human):Fc (human) (rec.) (non-lytic)

CHF 330.00
In stock
CHI-HF-22002-C05050 µgCHF 330.00
CHI-HF-22002-30503 x 50 µgCHF 825.00
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Product Details
Synonyms Interleukin-2; Aldesleukin
Product Type Protein
Properties
Source/Host CHO cells
Sequence

The extracellular domain of human IL-2 (aa 21-153) is fused to the N-terminus of the Fc region of a mutant human IgG1.

Crossreactivity Human
Biological Activity

Shows the biological function of the IL-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.

Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.06EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute in 50µl sterile water.
Add 1X PBS to the desired protein concentration.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (human) IgG1 Control (rec.) (non-lytic)

Other Product Data

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

NCBI reference NP_000577.2: IL-2 (human)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.

Product References
  1. Augmentation of immune responses to HIV-1 and simian immunodeficiency virus DNA vaccines by IL-2/Ig plasmid administration in rhesus monkeys: D.H. Barouch, et al.; PNAS 97, 4192 (2000)
  2. Control of viremia and prevention of clinical AIDS in Rhesus monkeys by cytokine-augmented DNA vaccination: D.H. Barouch, et al.; Science 290, 486 (2000)
  3. Vaccine-elicited immune responses prevent clinical AIDS in SHIV(89.6P)-infected rhesus monkeys: D.H. Barouch, et al.; Immunol. Lett. 79, 57 (2001)
  4. Spontaneous Resolution of Acute Rejection and Tolerance Induction With IL-2 Fusion Protein in Vascularized Composite Allotransplantation: R. Jindal, et al.; Am. J. Transplant. 15, 1231 (2015)
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