CD273 [PD-L2] (human):Fc (human) (recombinant protein) (non-lytic)

Specifications / Handling

More Information
Product Details
Synonyms	PD-L2; Programmed Cell Death 1 Ligand 2; PDCD1 Ligand 2; B7-DC; PDL2
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	The extracellular domain of human CD273 [PD-L2] (aa 20-219) is fused to the N-terminus of the Fc region of a mutant human IgG1.
Crossreactivity	Human
Biological Activity	Shows the biological function of the CD273 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
Purity	≥98% (SDS-PAGE)
Endotoxin Content	<0.06EU/μg protein (LAL test; Lonza).
Reconstitution	Reconstitute at 100μg/ml in sterile PBS.
Formulation	Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.) (non-lytic)
Other Product Data	Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). NCBI reference NP_079515.2: CD273 (human)
Declaration	Manufactured by Chimerigen.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

T cells require a signal induced by the engagement of the T cell receptor and a “costimulatory” signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. CD273 [PD-L2] is one of two ligands for programmed death-1 (PD-1; CD279), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L1. CD273 is broadly expressed and also up regulated in a variety of tumor cell lines. On previously activated T cells, CD273 interaction with PD-1 inhibits TCR mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. CD273 has a costimulatory function on resting T cells activated with suboptimal TCR signals.

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CD273 [PD-L2] (human):Fc (human) (rec.) (non-lytic)