HMGB1 (human) (rec.) (His)

CHF 330.00
In stock
CHI-HR-200HMGB1-C02525 µgCHF 330.00
More Information
Product Details
Synonyms High Mobility Group Protein B1
Product Type Protein
Source/Host E. coli

Human HMGB1 (aa 24-215) is fused at the N-terminus to a His-tag.

Crossreactivity Human
MW ~20-25kDa
Purity ≥90% (SDS-PAGE)
Endotoxin Content <1EU/mg protein (LAL test; Lonza).
Reconstitution Reconstituted in sterile H2O not less than 100μg/ml, which can then be further diluted in other aqueous solutions.
Formulation Lyophilized from a concentrated sterile solution containing 50mM Tris-Hcl buffer (pH 8.0) and 100mM NaCl.
Other Product Data

NCBI reference NP_002119.1: HMGB1 (human)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
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Product Specification Sheet
Datasheet Download PDF

HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-κB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-κB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis.

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