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Chimerigen
HMGB1 (human) (rec.) (His)
330
CHF
CHF 330.00
In stock
CHI-HR-200HMGB1-C02525 µgCHF 330.00
| Product Details | |
|---|---|
| Synonyms | High Mobility Group Protein B1 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Human HMGB1 (aa 24-215) is fused at the N-terminus to a His-tag. |
| Crossreactivity | Human |
| MW | ~20-25kDa |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <1EU/mg protein (LAL test; Lonza). |
| Reconstitution | Reconstituted in sterile H2O not less than 100μg/ml, which can then be further diluted in other aqueous solutions. |
| Formulation | Lyophilized from a concentrated sterile solution containing 50mM Tris-Hcl buffer (pH 8.0) and 100mM NaCl. |
| Other Product Data |
NCBI reference NP_002119.1: HMGB1 (human) |
| Declaration | Manufactured by Chimerigen. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-κB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-κB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis.
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