HMGB1 (rat):Fc (human) (recombinant protein)

Specifications / Handling

More Information
Product Details
Synonyms	High Mobility Group Protein B1
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	The extracellular domain of rat HMGB1 (aa 24-215) is fused to the N-terminus of the Fc region of human IgG1.
Crossreactivity	Rat
Purity	≥98% (SDS-PAGE)
Endotoxin Content	<0.06EU/μg protein (LAL test; Lonza).
Reconstitution	Reconstitute at 100μg/ml in sterile PBS.
Formulation	Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.)
Other Product Data	NCBI reference NP_037095.1: HMGB1 (rat)
Declaration	Manufactured by Chimerigen.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-κB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-κB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis.

Call	+41 61 926 6040
Fax	+41 61 926 6049

HMGB1 (rat):Fc (human) (rec.)