CD275 [B7-H2] (mouse):Fc (mouse) (rec.) (non-lytic)

CHF 430.00
In stock
CHI-MF-120B7H2-C100100 µgCHF 430.00
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Product Details
Synonyms B7-H2; ICOS Ligand; B7-like Protein Gl50; B7RP-1
Product Type Protein
Properties
Source/Host CHO cells
Sequence

The extracellular domain of mouse CD275 [B7-H2] (aa 48-279) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

Crossreactivity Mouse
Specificity

Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcγR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized human ICOS at 1μg/ml (100μl/well) can bind biotinylated B7-H2 (mouse):Fc (mouse) (rec.) (non-lytic) with a linear range of 0.1-1.0μg/ml. Optimal dilutions should be determined by each laboratory for each application.

Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.06EU/μg protein (LAL test; Lonza).
Reconstitution Reconstitute at 100μg/ml in sterile PBS.
Formulation Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control

Fc (mouse) IgG2a Control (rec.) (non-lytic)

Other Product Data

NCBI reference NP_056605.1: CD275 (mouse)

Declaration Manufactured by Chimerigen.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

Mouse CD275 (B7-H2) is a member of the growing B7 family of immune costimulatory proteins. CD275 has been identified as the ligand for ICOS, a member of the CD28 family of co-stimulatory receptors. Mouse CD275 is expressed on resting B cells and at low levels on monocytes. The CD275/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

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