Chimerigen

CD275 [B7-H2] (human) (rec.) (His)

CHF 390.00

In stock

CHI-HF-201B7H2-C05050 µgCHF 390.00

More Information
Product Details
Synonyms	B7-H2; ICOS Ligand; B7-like Protein Gl50; B7RP-1
Product Type	Protein
Properties
Source/Host	HEK 293 cells
Sequence	The extracellular domain of human CD275 [B7-H2] (aa 19-256) is fused at the C-terminus to a His-tag.
Crossreactivity	Human
Biological Activity	Measured by its binding ability in a functional ELISA.
MW	~40kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01EU/μg protein (LAL test; Lonza).
Reconstitution	Reconstitute 50µg vial with 100 µl sterile water to a concentration of 0.5mg/ml. Add 1X PBS to the desired protein concentration.
Formulation	Lyophilized from 0.2μm-filtered solution in PBS.
Other Product Data	NCBI reference NP_056074.1: CD275 (human)
Declaration	Manufactured by Chimerigen.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Human B7-H2 is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

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