Chimerigen

CD275 [B7-H2] (human):Fc (human) (rec.) (non-lytic)

CHF 450.00

In stock

CHI-HF-220B7H2-C100100 µgCHF 450.00

More Information
Product Details
Synonyms	B7-H2; ICOS Ligand; B7-like Protein Gl50; B7RP-1
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	The extracellular domain of human CD275 [B7-H2] (aa 1-256) is fused to the N-terminus of the Fc region of a mutant human IgG1.
Crossreactivity	Human
Specificity	Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcγR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
Biological Activity	Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3.
Purity	≥98% (SDS-PAGE)
Endotoxin Content	<0.06EU/μg protein (LAL test; Lonza).
Reconstitution	Reconstitute with 100 µl sterile water. Add 1X PBS to the desired protein concentration.
Formulation	Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control	Fc (human) IgG1 Control (rec.) (non-lytic)
Other Product Data	NCBI reference NP_056074.1: CD275 (human)
Declaration	Manufactured by Chimerigen.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Human B7-H2 is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

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