CD54 [ICAM-1] (mouse):Fc (mouse) (recombinant protein) (non-lytic)

Specifications / Handling

More Information
Product Details
Synonyms	ICAM1; Intercellular Adhesion Molecule 1
Product Type	Protein
Properties
Source/Host	CHO cells
Sequence	The extracellular domain of mouse CD54 [ICAM-1] (aa 28-485) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.
Crossreactivity	Mouse
Biological Activity	Shows the biological function of the CD54 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
Purity	≥98% (SDS-PAGE)
Endotoxin Content	<0.06EU/μg protein (LAL test; Lonza).
Reconstitution	Reconstitute at 100μg/ml in sterile PBS.
Formulation	Lyophilized from 0.2μm-filtered solution in PBS.
Protein Negative Control	Fc (mouse) IgG2a Control (rec.) (non-lytic)
Other Product Data	Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC). NCBI reference AAH08626.1: CD54 (mouse)
Declaration	Manufactured by Chimerigen.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac1. CD54 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-γ, TNF-α, IL-1β and LPS. Soluble CD54 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

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CD54 [ICAM-1] (mouse):Fc (mouse) (rec.) (non-lytic)