anti-8-Hydroxy-2'-deoxyguanosine [8-OHdG], mAb (N45.1)

CHF 992.00
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JAI-MOG-100P100 µgCHF 992.00
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Product Details
Product Type Monoclonal Antibody
Properties
Clone N45.1
Isotype Mouse IgG1κ
Source/Host Ascites
Immunogen/Antigen 8-Hydroxy-2’-deoxyguanosine (8OHdG) conjugated keyhole limpet hemocyanin.
Application Immunohistochemistry: Recommended concentration is 5-10μg/mL
ELISA
Crossreactivity All
Human
Specificity Recognizes 8-OHdG. Does not cross-react with 19 analogues of 8-OHdG (guanosine (G), 7-methylG, 6-SHG, 8-bromoG, dA, dC, dT, dI, dU, dG, O6-methyldG, 8-OHdA, guanine (Gua), O6-methylGua, 8-OHGua, uric acid, urea, creatine, creatinine). Minimal cross-reactivity with 8-sulfhydrylG and 8-OHG (less than 1%).
Purity Detail Ammonium sulfate purified.
Formulation Lyophilized. Contains 10mM PBS, pH7.4 containing 1.0% BSA.
Reconstitution Reconstitute with 1000μL of distilled water.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Declaration Manufactured by JaICA.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 3 years after receipt when stored at -20°C.
After reconstitution, prepare aliquots and store at -20°C.
Documents
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Product Specification Sheet
Datasheet Download PDF

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals (incl. singlet oxygen and direct photodynamic action) that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8-OHdG is popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA and can be detected in tissue, serum, urine and other biomaterials.

Product References
  1. Quantitative immunohistochemical determination of 8-hydroxy-2'deoxyguanosine by a monoclonal antibody N45.1: Its application to ferric nitrilotriacetate-induced renal carcinogenesis model: S. Toyokuni, et al.; Lab. Invest. 76, 365 (1997) [Specifity of the antibody clone N45.1] 
  2. 8-Hydroxy-2'deoxyguanosine is increased in epidermal cells of hairless mice after chronic ultraviolet B exposure: Y. Hattori, et al.; J. Invest. Dermatol. 107, 733 (1996) [Immunohistochemistry of UV-B irradiated mouse skin] 
  3. Induction and nuclear translocation of thioredoxin by oxidative damage in the mouse kidney: independence of tubular necrosis and sulfhydryl depletion: T. Tanaka, et al.; Lab. Invest. 77, 145 (1997) [Immunohistochemistry of ferric nitrotriacetate-treated mouse kidney] 
  4. Hyperglycemia causes oxidative stress in pancreatic -cells of GK rats, a model of type 2 diabetes: Y. Ihara, et al.; Diabetes 48, 927 (1999) [Immunohistochemistry of pancreatic islets from GK rat, a diabetes model] 
  5. DNA oxidation injury in bone early after steroid administration is involved in the pathogenesis of steroid-induced osteonecrosis: T. Ichiseki, et al.; Rheumatology 44, 456 (2005) [Immunohistochemistry of rabbit tissue] 
  6. Protective effect of persimmon (Diospyros kaki) peel proanthocyanidin against oxidative damage under H2O2 -induced cellular senescence: Y.A. Lee, et al.; Biol. Pharm. Bull. 31, 1265 (2008) [Application to human cultured cells]
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