Nuclease P1 (from Penicillium citrinum)

CHF 702.00
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JAI-OPNUCP1500 UnitsCHF 702.00
 
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Product Details
Product Type Protein
Properties
Sequence A zinc dependent glycoprotein consisting of 270 amino acid residues.
Application Used for DNA samples pretreatment to detect 8-OHdG.
Biological Activity 3′-5′-Phosphodiesterase: One unit will liberate ~1.0μmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3′-Nucleotidase: One unit will hydrolyze ~4.0μmol of orthophosphate from 3′-AMP per min at pH 7.2 at 37°C.
MW 42-50 kDa
Formulation Lyophilized.
Other Product Data The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

Optimal pH:
5.3 (RNA and heat-denatured DNA); 7.2 (3'-AMP)

Heat Stability: Stable below 60°C at pH 5.3 for 30 minutes.
Declaration Manufactured by JaICA.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Use/Stability Stable for at least 1 year after receipt when stored at +4°C.
Documents
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Product Specification Sheet
Datasheet Download PDF
Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phospho-oligonucleotides. Nuclease P1 hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat-denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mmol/l of sodium chloride at pH 6.0.
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