Nuclease P1 (from <i>Penicillium citrinum</i>)

Specifications / Handling

More Information
Product Details
Product Type	Protein
Properties
Sequence	A zinc dependent glycoprotein consisting of 270 amino acid residues.
Application	Used for DNA samples pretreatment to detect 8-OHdG.
Biological Activity	3′-5′-Phosphodiesterase: One unit will liberate ~1.0μmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3′-Nucleotidase: One unit will hydrolyze ~4.0μmol of orthophosphate from 3′-AMP per min at pH 7.2 at 37°C.
MW	42-50 kDa
Formulation	Lyophilized.
Other Product Data	The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8. Optimal pH: 5.3 (RNA and heat-denatured DNA); 7.2 (3'-AMP) Heat Stability: Stable below 60°C at pH 5.3 for 30 minutes.
Declaration	Manufactured by JaICA.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	+4°C
Use/Stability	Stable for at least 1 year after receipt when stored at +4°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phospho-oligonucleotides. Nuclease P1 hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat-denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mmol/l of sodium chloride at pH 6.0.

Product References

The interactions of dopamine and oxidative damage in the striatum of patients with neurodegenerative diseases: H. Li, et al.; J. Neurochem. 152, 235 (2020)

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Nuclease P1 (from Penicillium citrinum)