Nuclease P1 (from Penicillium citrinum)
|Sequence||A zinc dependent glycoprotein consisting of 270 amino acid residues.|
|Application||Used for DNA samples pretreatment to detect 8-OHdG.|
|Biological Activity||3′-5′-Phosphodiesterase: One unit will liberate ~1.0μmol of acid soluble nucleotides from RNA per min at pH 5.3 at 37°C. 3′-Nucleotidase: One unit will hydrolyze ~4.0μmol of orthophosphate from 3′-AMP per min at pH 7.2 at 37°C.|
|Other Product Data||
The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.
5.3 (RNA and heat-denatured DNA); 7.2 (3'-AMP)
Heat Stability: Stable below 60°C at pH 5.3 for 30 minutes.
|Declaration||Manufactured by JaICA.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||+4°C|
|Use/Stability||Stable for at least 1 year after receipt when stored at +4°C.|
|Product Specification Sheet|
Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phospho-oligonucleotides. Nuclease P1 hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat-denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mmol/l of sodium chloride at pH 6.0.
- The interactions of dopamine and oxidative damage in the striatum of patients with neurodegenerative diseases: H. Li, et al.; J. Neurochem., ahead of print (2019)