Proteins

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  1. SUMO1 (human) (rec.) (Rhodamine 110)
    SBB-PS0028
    SBB-PS0028-C05050 µg
    CHF 434.00
    Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 200nM, 100nM, 50nM SUMO1-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH 7.5, 1mM TCEP, 0.1mg/ml BSA.
  2. SUMO2 (human) (rec.) (Rhodamine 110)
    SBB-PS0029
    SBB-PS0029-C05050 µg
    CHF 434.00
    Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 200nM, 100nM, 50nM SUMO2-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH 7.5, 1mM TCEP, 0.1mg/ml BSA.
  3. TDO (Anopheles gambiae) (rec.) (His)
    AG-40T-0101
    AG-40T-0101-C05050 µg
    CHF 590.00
  4. UcH-L3 (human) (rec.) (untagged)
    SBB-DE0023
    SBB-DE0023-C05050 µg
    CHF 278.00
    Michaelis–Menten Kinetics: Ubiquitin Rhodamine 110 serially diluted from 1.6 to 0.05µM was digested with 30pM UcHL3 over time. The assay was carried out in a reaction buffer of 50mM HEPES pH 7.5, 100mM NaCl, 1mM TCEP, 0.1mg/ml BSA, at 25°C. Initial

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