RevMab

anti-SARS-CoV-2 Nucleocapsid, Rabbit Monoclonal (RM420)

CHF 459.00
In stock
REV-31-1307-00-C100100 µgCHF 459.00
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Product Details
Synonyms 2019-nCoV N Protein
Product Type Recombinant Antibody
Properties
Clone RM420
Isotype Rabbit IgG
Source/Host Rabbit
Immunogen/Antigen A recombinant 2019-nCov Nucleocapsid protein expressed in E.coli.
Application

Sandwich ELISA: Used as a capture antibody, paired with anti-2019-nCov NP chimeric human monoclonal antibody, clone RMH03. The binding activity of N protein with anti-N protein antibody was verified with the sensitivity of 0.1-0.3ng/mL.

Crossreactivity Virus
Specificity

This antibody reacts to 2019-nCov Nucleocapsid protein.

Purity Protein A purified.
Purity Detail Protein A affinity purified from an animal origin-free culture supernatant.
Concentration 1mg/ml
Formulation Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Accession Number P59595
Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. The coronaviral genome encodes four major structural proteins: the Spike (S) protein, Nucleocapsid (N) protein, Membrane/Matrix (M) protein and the Envelope (E) protein. The SARS Envelope (E) protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. The SARS Membrane/Matrix (M) protein is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with SARS Spike (S) protein and the Nucleocapsid (N) protein. The CoV Spike (S) protein assembles as trimer and plays the most important role in viral attachment, fusion and entry. It is composed of a short intracellular tail, a transmembrane anchor and a large ectodomain that consists of a receptor binding S1 subunit (RBD domain) and a membrane-fusing S2 subunit. Sequence analysis of the SARS-CoV-2 S protein genome showed that it was only 75% identical with the SARS-CoV S protein. However, analysis of the receptor binding motif (RBM) in the S protein showed that most of the amino acid residues essential for receptor binding were conserved between SARS-CoV and SARS-CoV-2, suggesting that the 2 CoV strains use the same host receptor for cell entry, the receptor angiotensin-converting enzyme 2 (ACE2). The N protein contains two domains, both of them bind the virus RNA genome via different mechanisms.

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