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AdipoGen Life Sciences
anti-SARS-CoV-2 Spike Protein S1, mAb (rec.) (blocking) (Covi-1) (preservative free)
370
CHF
CHF 370.00
In stock
AG-27B-6005PF-C100100 µgCHF 370.00
Figure 1: Binding of human ACE2 to the Spike protein of SARS-CoV-2 is inhibited by the antibody SARS-CoV-2 Spike Protein S1, mAb (rec.) (blocking) (Covi-1) (preservative free) (Prod. No. AG-27B-6005PF).
Methods: SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) (Prod. No. AG-40B-0194) is coated on an ELISA plate at 1µg/ml. SARS-CoV-2 Spike Protein S1, mAb (rec.) (blocking) (Covi-1) (preservative free) (Prod. No. AG-27B-6005PF) or an unrelated recombinant mAb (Control) is added (starting at 40µg/ml with a two-fold serial dilution) together with 500ng/µl of ACE2 (human) (rec.) (Biotin) (Prod. No. AG-40B-0192B). After incubation for 1h at 37°C, the binding is detected using Streptavidin (HRP).
Methods: SARS-CoV-2 Spike Protein S1 (RBD):Fc (human) (rec.) (Prod. No. AG-40B-0194) is coated on an ELISA plate at 1µg/ml. SARS-CoV-2 Spike Protein S1, mAb (rec.) (blocking) (Covi-1) (preservative free) (Prod. No. AG-27B-6005PF) or an unrelated recombinant mAb (Control) is added (starting at 40µg/ml with a two-fold serial dilution) together with 500ng/µl of ACE2 (human) (rec.) (Biotin) (Prod. No. AG-40B-0192B). After incubation for 1h at 37°C, the binding is detected using Streptavidin (HRP).
Figure 2: ELISA binding assay with SARS-CoV-2 S1 Protein (RBD) (rec.) (His) (Prod. No. CHI-B232004) using anti-SARS-CoV-2 Spike Protein S1, mAb (rec.) (Covi-1) (preservative free) (#AG-27B-6005PF) (blue line) and an unrelated antibody as negative control (red line).
| Product Details | |
|---|---|
| Synonyms | 2019-nCoV Spike Protein S1 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | Covi-1 |
| Isotype | Human IgG1 |
| Source/Host | HEK 293 cells |
| Immunogen/Antigen | Recombinant SARS-CoV-2 Spike Protein S1 receptor binding domain. |
| Label/Conjugates | Preservative Free |
| Application |
ELISA Binding Assay Functional Application: Blocking Western Blot: Works only in non-denatured conditions (1/1’000). |
| Crossreactivity | Virus |
| Specificity |
Recognizes and binds to recombinant SARS-CoV-2 Spike Protein S1. It blocks the interaction of SARS-CoV-2 Spike Protein S1 (RBD) to human ACE2. Detects a different epitope as anti-SARS-CoV-2 Spike Protein S1, mAb (rec.) (blocking) (Covi-2) (Prod. No. AG-27B-6006PF). |
| Purity | ≥98% (SDS-PAGE) |
| Purity Detail | Protein A purified. |
| Endotoxin Content | <0.001EU/μg |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
Uniprot P0DTC2: SARS-CoV-2 |
| Accession Number | P0DTC2 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Coronaviruses (CoVs) are enveloped non-segmented positive-sense single-stranded RNA viruses and can infect respiratory, gastrointestinal, hepatic and central nervous system of human and many other wild animals. Recently, a new severe acute respiratory syndrome β-coronavirus called SARS-CoV-2 (or 2019-nCoV) has emerged, which causes an epidemic of acute respiratory syndrome (called coronavirus human disease 2019 or COVID-19).
SARS-CoV-2 shares 79.5% sequence identity with SARS-CoV and is 96.2% identical at the genome level to the bat coronavirus BatCoV RaTG133, suggesting it had originated in bats. SARS-CoV-2 contains 4 structural proteins, including Envelope (E), Membrane (M), Nucleocapsid (N) and Spike (S), which is a transmembrane protein, composed of two subunits S1 and S2. The S1 subunit contains a receptor binding domain (RBD), which binds to the cell surface receptor Angiotensin-Converting Enzyme 2 (ACE2) present at the surface of epithelial cells, causing mainly infection of human respiratory cells.
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