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RevMab
anti-5-Formylcytosines (5-fC), Rabbit Monoclonal (RM477)
600
CHF
CHF 600.00
In stock
REV-31-1369-00-C05050 µgCHF 600.00
Figure 1: ELISA of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477 (1 ug/mL). The plate was coated with different concentrations of linear denatured DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or 5-formylcytosines (5-fC).
| Product Details | |
|---|---|
| Synonyms | 5-fC |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | RM477 |
| Isotype | Rabbit IgG |
| Source/Host | Rabbit |
| Immunogen/Antigen | KLH-conjugated 5-Formylcytosine. |
| Application |
DotBlot: 0.5 µg/ml -2 µg/ml |
| Crossreactivity | All |
| Specificity |
RM477 reacts to 5-Formylcytosine (5-fC) in DNA. No cross-reactivity with non-modified cytosine and other modified cytosines in DNA. |
| Purity | Protein A purified. |
| Purity Detail | Protein A affinity purified from an animal origin-free culture supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | N/A |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
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