RevMab

5-Formylcytosines (5-fC) DNA Matched Antibody Pair

CHF 777.00
In stock
REV-31-1369-MK-KI011 SetCHF 777.00
More Information
Product Details
Synonyms 5-fC
Product Type Set
Properties
Application Set Quantitative ELISA
Specificity

This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.

Crossreactivity All
Quantity

ELISA:
Capture Antibody 50-200ng/well
Detection Antibody 0.1-0.5μg/mL

Assay Type Sandwich
Detection Type Colorimetric
Kit Contains

1) Capture Antibody: Anti-5-Formylcytosine (5-fC) Rabbit Monoclonal Antibody, Clone RM477, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.
2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Manual No
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.

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