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RevMab
5-Formylcytosines (5-fC) DNA Matched Antibody Pair
777
CHF
CHF 777.00
In stock
REV-31-1369-MK-KI011 SetCHF 777.00
Figure 1: Detection of 5-Formylcytosines (5-fC) in linear denatured DNA (426 bp). Sandwich ELISA using anti-Formylcytosines (5-fC) (clone RM477) as the capture antibody (50ng/well at 1ug/mL), and biotinylated anti-Adenine and Guanine (clone RM476) as the detection antibody (0.2ug/mL), followed by an AP conjugated streptavidin.
| Product Details | |
|---|---|
| Synonyms | 5-fC |
| Product Type | Set |
| Properties | |
| Application Set | Quantitative ELISA |
| Specificity |
This antibody pair detects 5-Formylcytosines (5-fC) in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-hmC, or 5-caC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays. |
| Crossreactivity | All |
| Quantity |
ELISA: |
| Assay Type | Sandwich |
| Detection Type | Colorimetric |
| Kit Contains |
1) Capture Antibody: Anti-5-Formylcytosine (5-fC) Rabbit Monoclonal Antibody, Clone RM477, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Declaration | Manufactured by RevMab Biosciences. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Manual | No |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
5-Formylcytosine (5fC) is a pyrimidine nitrogen base derived from cytosine and a recently discovered modification of DNA. Just as 5-hydroxymethylcytosine (5-hmC) is generated when the Tet family of cytosine oxygenase enzymes converts 5-methylcytosine (5-mC), it has been shown that continued activity of the Tet proteins on hydroxymethylcytosine will generate 5-formylcytosine (5-fC) and 5-carboxylcytosine. It is believed that this conversion process may represent a pathway of DNA demethylation.
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