RevMab

anti-CD45 (human), Rabbit Monoclonal (RM497)

CHF 459.00
In stock
REV-31-1389-00-R100100 µlCHF 459.00
More Information
Product Details
Synonyms Receptor-type tyrosine-protein Phosphatase C; Leukocyte Common Antigen; L-CA; T200
Product Type Recombinant Antibody
Properties
Clone RM497
Isotype Rabbit IgG
Source/Host Rabbit
Immunogen/Antigen Recombinant human CD45 (Receptor-type tyrosine-protein phosphatase C).
Application

Immunohistochemistry (IHC): 1:100-1:200 dilution
Western Blot (WB): 1:200-1:500 dilution

Crossreactivity Human
Specificity

This antibody reacts to human CD45 (Receptor-type tyrosine-protein phosphatase C).

Purity Protein A purified.
Purity Detail Protein A affinity purified from an animal origin-free culture supernatant.
Concentration N/A
Formulation Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide.
Isotype Negative Control

Rabbit IgG

Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.

Accession Number P08575
Declaration Manufactured by RevMab Biosciences.
Shipping and Handling
Shipping BLUE ICE
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF
Description

CD45 (LCA; Leukocyte Common Antigen; Protein tyrosine phosphatase, receptor type, C; PTPRC) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, which differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions. Antibodies against the different isoforms of CD45 are used in routine immunohistochemistry to differentiate between immune cell types, as well as to differentiate between histological sections from lymphomas and carcinomas.

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