Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (His)

CHF 179.00
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SBB-CE0058-C05050 µgCHF 179.00
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Product Details
Synonyms Ubiquitin E1 Enzyme; Ubiquitin-like Modifier-activating Enzyme 1; A1S9T; AMCX1; GXP1; POC20; SMAX2
Product Type Protein
Properties
Source/Host Sf21 cells
Sequence

Human UBA1 (aa1-2-1058) (Accession Nr. P22314) is fused at the N-terminus to a His-tag.

Crossreactivity Human
Application

Typical working concentration range is 10-100nM.

MW ~120kDa
Purity ≥95% (SDS-PAGE)
Concentration Lot dependent.
Accession Number P22314
Formulation Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP.
Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -80°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSDS No
Product Specification Sheet
Datasheet Download PDF

UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1's active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzyme's active site cysteine.

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