SouthBayBio

Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (His)

CHF 291.00

In stock

SBB-CE0058-C05050 µgCHF 291.00

Figure 1: SDS-PAGE: From left to right, increasing amounts of UBA1 (Prod. No. SBB-CE0058) loaded onto a 4-12% SDS-PAGE gel, stained with Coomassie blue.

Specifications / Handling

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Product Details
Synonyms	Ubiquitin E1 Enzyme; Ubiquitin-like Modifier-activating Enzyme 1; A1S9T; AMCX1; GXP1; POC20; SMAX2
Product Type	Protein
Properties
Source/Host	Sf21 cells
Sequence	Human UBA1 (aa12-1058) (Accession Nr. P22314) is fused at the N-terminus to a His-tag.
Crossreactivity	Human
Application	Typical working concentration range is 10-100nM.
MW	~120kDa
Purity	≥95% (SDS-PAGE)
Concentration	Lot dependent.
Accession Number	P22314
Formulation	Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP.
Other Product Data	Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturers product datasheet.
Declaration	Manufactured by South Bay Bio.
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-80°C
Long Term Storage	-80°C
Handling Advice	Aliquot to avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -80°C.
Documents
MSDS	Inquire
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1's active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzyme's active site cysteine.

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