20S Immunoproteasome Assay Kit

CHF 772.00
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SBB-KP0037-KI011 KitCHF 772.00
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Product Details
Product Type Kit
Application Set Compound Screening

Readout: Endpoint/Kinetic
Label or Dye: 7-Amino-4-methylcoumarin
Detection Method: Fluorescence
Substrate Properties: Protein-based Substrate.
Excitation/Emission (nm): 345/445

Crossreactivity Human

Sufficient Reagents for:
100 x 50 μl reactions

Detection Type Fluorescent
Kit Contains

10x 20S Immunoproteasome
50x LLVY-AMC, Chemotrypsin-like activity
50x PAL-AMC, β1i/PSMB9 specific substrate
50x ANW-AMC, β5i/PSMB8 specific substrate
50x Inhibitor (ONX-0914, 2mM) in 100% DMSO
10x Reaction Buffer
50x SDS(1.75%) in H20
100x free AMC Standard (40μM)

Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Manual No
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits β1i/ PSMB9 (PAL-AMC) and β5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit β5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.

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