20S Constitutive Proteasome Assay Kit
|Application Set||Compound Screening|
Sufficient Reagents for:
10x 20S Proteasome
|Other Product Data||
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-80°C|
Aliquot to avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
|Product Specification Sheet|
South Bay Bio's kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits β1/PSMB6 (LLE-AMC) and Chymotrypsin-like activity of β5/PSMB5 (WLA-AMC). Additionally, the compound MG-132 is included, which can be used to inhibit the proteasome. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission=445nm; allowing for a real-time read out of 20S proteasome specific activity.