20S Constitutive Proteasome Assay Kit

CHF 425.00
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SBB-KP0038-KI011 KitCHF 425.00
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Product Details
Product Type Kit
Properties
Application Set Compound Screening
Specificity

Readout: Endpoint/Kinetic
Label or Dye: 7-Amino-4-methylcoumarin
Detection Method: Fluorescence
Substrate Properties: Protein-based Substrate.
Excitation/Emission (nm): 345/445

Crossreactivity Human
Quantity

Sufficient Reagents for:
100 x 50 μl reactions

Detection Type Fluorescent
Kit Contains

10x 20S Proteasome
50x LLVY-AMC, Chemotrypsin-like activity
50x WLA-AMC, β5/PSMB5 specific substrate
50x LLE-AMC, β1/PSMB6 specific substrate
50x Inhibitor (MG-132, 2mM) in 100% DMSO
10x Reaction Buffer
50x SDS(1.75%) in H20
100x free AMC Standard (40μM)

Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.

Declaration Manufactured by South Bay Bio.
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice Aliquot to avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Documents
Manual No
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

South Bay Bio's kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits β1/PSMB6 (LLE-AMC) and Chymotrypsin-like activity of β5/PSMB5 (WLA-AMC). Additionally, the compound MG-132 is included, which can be used to inhibit the proteasome. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission=445nm; allowing for a real-time read out of 20S proteasome specific activity.

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