Ubiquitin propargylamide (human) (rec.)
SBB-PS0034-C05050 µgCHF 194.00
Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 15.0nM and 0.1nM Ubiquitin propargylamide (SBB-PS0034).
|Sequence||Human ubiquitin (aa1-76) (Accession Nr. P0CG47) with a C-terminal propargylamide group.|
|Application||Typical working concentration range is 1-5 μM. Reaction conditions will need to be optimized for each specific application.|
|Formulation||Liquid. In 50mM MES pH 6.0, 100mM sodium chloride.|
|Other Product Data||
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.
|Declaration||Manufactured by South Bay Bio.|
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-80°C|
|Handling Advice||Aliquot to avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -80°C.|
|Product Specification Sheet|
Ubiquitin is a small (8.5kDa) regulatory protein that has been found in almost all tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or ubiquitylation. Ubiquitination can affect proteins in many ways: it can signal for their degradation via the proteasome, alter their cellular location, affect their activity and promote or prevent protein interactions. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various linkage specificities. This product consists of a full-length human, mature ubiquitin polypeptide (amino acids 1-76), expressed in E.coli with a C-terminal warhead (Propargylamide). Ubiquitin propargylamide is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic propargylamide group. Ubiquitin propargylamide can be used for activity profiling experiments and determining DUB inhibitor specificity. It targets three of the four major DUB families: UCH (Ubiquitin C-terminal hydrolases), USP (Ubiquitin specific proteases), OTU (Ovarian tumor proteases) and MJD (Machado-Josephin domain proteases) while JAMM metalloproteases are not inhibited.