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SouthBayBio
DDB1/CRBN Complex (human) (rec.) (untagged)
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Figure 1: DDB1/CRBN SDS-PAGE. From left to right, increasing amounts of DDB1/CRBN were loaded onto a 4-20% SDS-PAGE gel, stained with Coomassie brillant blue. Purity is >98%.
| Product Details | |
|---|---|
| Synonyms | DNA Damage-binding Protein 1/Cereblon |
| Product Type | Protein |
| Properties | |
| Source/Host | Sf9 cells |
| Sequence |
Recombinant human DDB1 (1-1140) with CRBN (2-442) complex is untagged. |
| Crossreactivity | Human |
| Application |
Typical working concentrations of this enzyme range from 20-50nM in combination with Cul4a/Rbx1 (SBB-SC0115) for ubiquitination assays. |
| MW | DDB1 (~127kDa), CRBN (~51kDa) |
| Purity | ≥98% (SDS-PAGE) |
| Concentration | Lot dependent. |
| Accession Number | Q16531: DDB1 / Q96SW2: CRBN |
| Formulation | Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol, 1mM TCEP |
| Other Product Data |
Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. |
| Declaration | Manufactured by South Bay Bio. |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -80°C |
| Long Term Storage | -80°C |
| Handling Advice | Aliquot to avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -80°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediates the ubiquitination of numerous target proteins, such as ILF2 and MEIS2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. In vivo, the endogenous substrate of CRBN MEIS2 is blocked from binding by immunomodulatory drugs (IMiDs), i.e. Thalidomide, which instead promotes the recruitment of IKZF1 or IKZF3 for degradation. DDB1 and CRBN complex has been coexpressed and purified from insect cells Sf9.
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