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Vector
VECTASHIELD PLUS Antifade Mounting Medium with DAPI
80
CHF
CHF 80.00
In stock
VC-H-2000-L0022 mlCHF 80.00
VC-H-2000-L01010 mlCHF 278.00
Human colon stained with rabbit anti-Cytokeration (AE1/AE3) and mouse anti-Desmin, both detected simultaneously with VectaFluor Duet Double Labeling Kit (VC-DK-8828); Dylight488 (green)/Dylight594 (red). Mounted in Vectashield Plus with DAPI (nuclei, blue).
| Product Details | |
|---|---|
| Product Type | Chemical |
| Properties | |
| Crossreactivity | Multi-species |
| Declaration | Manufactured by Vector Laboratories |
| Other Product Data |
VECTASHIELD PLUS Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes. VECTASHIELD PLUS provides several advantages over the regular VECTASHIELD (VC-H-1000 & VC-H-1200): it produces a lower blue background upon initial viewing. An inherent blue tone using the regular VECTASHIELD can be resolved by illuminating the slide for 45 seconds at a higher power objective, such as 40x. VECTASHIELD PLUS contains superior signal retention in the far-red spectrum (e.g. Cy5), unlike quenching of far-red fluorophores using the regular VECTASHIELD. The specimen can be imaged 1 hour after coverslip application. The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD PLUS Mounting Medium without DAPI (VC-H1900). DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm, and is ideal as a nuclear or chromosomal counterstain. Click here for Original Manufacturer Product Datasheet and Complete Product Information |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +20°C |
| Long Term Storage | +4°C |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
VECTASHIELD PLUS Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. This medium inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes. The features of this product include, no inherent toning or background, superior signal retention across the spectrum from blue to far red, mounted sections can be viewed in 1 hour, superior non-setting formulation and DAPI (blue) counterstain premixed with medium. The original VECTASHIELD PLUS Mounting Medium with DAPI does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 2-8 °C, protected from light.
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